Buch, Englisch, 250 Seiten, Previously published in hardcover, Format (B × H): 152 mm x 229 mm, Gewicht: 363 g
Buch, Englisch, 250 Seiten, Previously published in hardcover, Format (B × H): 152 mm x 229 mm, Gewicht: 363 g
ISBN: 978-1-61737-306-0
Verlag: Humana Press
In this revised and expanded second edition, seasoned experts describe in step-by-step detail their best state-of-the-art techniques for studying neuronal cell death. These readily reproducible methods solve a wide variety of research problems, including the detection of the key proteins involved in neuronal apoptosis (Bax protein, cytochrome c, and caspases), the direct assessment of the role of pro-apoptotic proteins in neurons by viral infections and microinjections, and the detection of pro-apoptotic proteins in situ. There are also hands-on methods for the study of apoptosis mechanisms in neuronal compartments, for studying synaptosis, and for establishing gene expression profiles in neurodegenerative brain tissues by using DNA microarrays.
Zielgruppe
Research
Autoren/Hrsg.
Fachgebiete
Weitere Infos & Material
Molecular Characterization of the Proapoptotic Protein Bax.- Assessing Cytochrome-c Release from Mitochondria.- Monitoring Caspases in Neuronal Cell Death.- HSV Amplicon Vectors in Neuronal Apoptosis Studies.- Microinjections to Study the Specific Role of Proapoptotic Proteins in Neurons.- Designing Microarray Experiments for Neurobiology.- Analysis of Gene Expression by Nylon Membrane cDNA Arrays.- Glyceraldehyde-3-phosphate Dehydrogenase as a Target for Antiapoptotic Drugs.- Analytical Approaches for Investigating Apoptosis and Other Biochemical Events in Compartmented Cultures of Sympathetic Neurons.- Detection and Analysis of Synaptosis.- In Situ Detection of Apoptotic Neurons.- Apoptotic and Oxidative Indicators in Alzheimer’s Disease.
