Protein and Peptide Analysis by LC-MS

Experimental Strategies
Erscheinungsjahr 2011
ISBN: 978-1-84973-182-9
Verlag: RSC Publishing

Buch, Englisch, 194 Seiten, Format (B × H): 159 mm x 244 mm, Gewicht: 513 g

This book is the first example in presenting LC-MS strategies for the analysis of peptides and proteins with detailed information and hints about the needs and problems described from experts on-the-job. The best advantage is -for sure- the practical insight of experienced analysts into their novel protein analysis techniques. Readers starting in 'Proteomics' should be able to repeat each experiment with own equipment and own protein samples, like clean-up, direct protein analysis, after (online) digest, with modifications and others. Furthermore, the reader will learn more about strategies in protein analysis, like quantitative analysis, industrial standards, functional analysis and more.

Autoren/Hrsg.

Letzel, Thomas

Fachgebiete

• Naturwissenschaften Chemie Analytische Chemie Instrumentelle Analytik, Chromatographie
• Naturwissenschaften Biowissenschaften Biochemie (nichtmedizinisch)

Inhaltsverzeichnis

Sample Preparation;

How to Couple and Handle HPLC and Mass Spectrometry;

LC-MS of Proteins;

LC-MS of Membrane Proteins;

LC-MS of Hydrolyzed Proteins;

Online-digest-LC-MS of Proteins;

Quantitative LC-MS of Proteins;

Top-Down Analysis;

LC-MS for the Identification of posttranslational modifications on proteins;

Functional Analysis of Proteins by LC-MS;

Bioinformatical Tools for the LC-MS Analysis of Proteins;

Industrial Standards and Strategies in LC-MS Analysis of Proteins

Über Autor(innen)

Letzel, Thomas
Dr Thomas Letzel is Head of Analytical Research at Munich Technical University.

Thomas Letzel is associate professor for analytical chemistry and bioanalysis at the Wissenschaftszentrum Weihenstephan (WZW) located at the TUM (Technische Universitõt M³nchen), Germany. He obtained his PhD in Chemistry with studies about soot aerosols and environmental analysis at the Chair for Analytical Chemistry of TUM. After a postdoc time in Amsterdam, The Netherlands, at the VU Amsterdam with topics in pharmaceutical and enzymatic analysis, he came back to TUM and was employed as a scientist, leading a new analytical research group with focus on bioanalysis. In latter position he got his Habilitation. He developed and established mass spectrometric techniques coupled with modern liquid phase solution techniques in enzymology, studying noncovalent complexes and observing molecular fingerprints. His research of novel mass spectrometric techniques comprises the identification and characterization of organic molecules in applications of biological, environmental, pharmaceutical, food, plant and nutrition sources.