Buch, Englisch, Band 112, 601 Seiten, Format (B × H): 160 mm x 236 mm, Gewicht: 1081 g
Reihe: Methods in Molecular Biology
Buch, Englisch, Band 112, 601 Seiten, Format (B × H): 160 mm x 236 mm, Gewicht: 1081 g
Reihe: Methods in Molecular Biology
ISBN: 978-0-89603-524-9
Verlag: Springer
Zielgruppe
Research
Autoren/Hrsg.
Fachgebiete
- Naturwissenschaften Chemie Organische Chemie Biochemie
- Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Medizin, Gesundheitswesen Biomedizin, Medizinische Forschung, Klinische Studien
- Naturwissenschaften Biowissenschaften Biochemie (nichtmedizinisch)
- Naturwissenschaften Biowissenschaften Zellbiologie
- Naturwissenschaften Biowissenschaften Proteinforschung
- Naturwissenschaften Biowissenschaften Biowissenschaften Genetik und Genomik (nichtmedizinisch)
Weitere Infos & Material
2-D Protein Gel Electrophoresis.- Solubilization of Proteins in 2-D Electrophoresis.- Preparation of Escherichia coli Samples for 2-D Gel Analysis.- Preparing 2-D Protein Extracts from Yeast.- 2-D Protein Extracts from Drosophila melanogaster.- Preparing 2-D Protein Extracts from Caenorhabditis elegans.- Eukaryotic Cell Labeling and Preparation for 2-D.- Differential Detergent Fractionation of Eukaryotic Cells.- Fractionated Extraction of Total Tissue Proteins from Mouse and Human for 2-D Electrophoresis.- Preparation and Solubilization of Body Fluids for 2-D.- 2-D Electrophoresis of Plant Proteins.- Quantifying Protein in 2-D PAGE Solubilization Buffers.- Measuring the Radioactivity of 2-D Protein Extracts.- Advantages and Disadvantages of Carrier Ampholyte IEF.- 2-D Electrophoresis Using Carrier Ampholytes in the First Dimension (IEF).- Nonequilibrium pH Gel Electrophoresis (NEPHGE).- High-Resolution, 2-D Protein Electrophoresis Using Nondedicated Equipment.- Large-Gel 2-D Electrophoresis.- Advantages of Immobilized pH Gradients.- Casting Immobilized pH Gradients (IPGs).- Analytical IPG-Dalt.- IPG-Dalt of Very Alkaline Proteins.- Running Preparative Carrier Ampholyte and Immobilized pH Gradient IEF Gels for 2-D.- In-Gel Sample Rehydration of Immobilized pH Gradient.- High-Resolution, IPG-Based, Mini Two-Dimensional Gel Electrophoresis.- Horizontal SDS-PAGE for IPG-Dalt.- Casting and Running Vertical Slab-Gel Electrophoresis for 2D-PAGE.- Nonreducing 2-D Polyacrylamide Gel Electrophoresis.- 2-D Diagonal Gel Electrophoresis.- 2-D Phosphopeptide Mapping.- Internal Standards for 2-D.- Autoradiography of 2-D Gels.- Double-Label Analysis.- Silver Staining of 2-D Electrophoresis Gels.- Staining of Preparative 2-D Gels.- Electroblotting of Proteins from 2-D Polyacrylamide Gels.- Detection of Total Proteins on Western Blots of 2-D Polyacrylamide Gels.- Protein Detection Using Reversible Metal Chelate Stains.- Glycoprotein Detection of 2-D Separated Proteins.- Image Acquisition in 2-D Electrophoresis.- Computer Analysis of 2-D Images.- 2-D Databases on the World Wide Web.- Comparing 2-D Electrophoretic Gels Across Internet Databases.- Constructing a 2-D Database for the World Wide Web.- Absolute Quantitation of 2-D Protein Spots.- Generating a Bacterial Genome Inventory.- Immunoaffinity Identification of 2-DE Separated Proteins.- 2-DE Spot Amino Acid Analysis with 9-Fluorenylmethyl Chloroformate.- N-Terminal Amino Acid Sequencing of 2-DE Spots.- Characterizing Proteins from 2-DE Gels by Internal Sequence Analysis of Peptide Fragments.- Obtaining Molecular Weights of Proteins and Their Cleavage Products by Directly Combining Gel Electrophoresis with Mass Spectrometry.- Identification of Proteins by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Using Peptide and Fragment Ion Masses.- Sample Preparation Methods for Mass Spectrometric Peptide Mapping Directly from 2-DE Gels.- Protein Identification and Analysis Tools in the ExPASy Server.- Automated Protein Identification Using Microcolumn Liquid Chromatography-Tandem Mass Spectrometry.- Peptide Sequencing of 2-DE Gel-Isolated Proteins by Nanoelectrospray Tandem Mass Spectrometry.