Buch, Englisch, 332 Seiten, Format (B × H): 178 mm x 254 mm
Reihe: Methods in Molecular Biology
Buch, Englisch, 332 Seiten, Format (B × H): 178 mm x 254 mm
Reihe: Methods in Molecular Biology
ISBN: 978-1-0716-5750-8
Verlag: Springer Us
This detailed volume explores approaches used for identifying new protein therapeutics followed by methods designed to define the therapeutic functions and molecular targets. Cell and preclinical animal models of disease are then assessed for responses to newer protein reagents, including methods from molecular and cellular assays, animal models, and AI analysis of protein interactions. Finally, clinical trials allow for final testing of protein therapeutic safety and efficacy, allowing a final assessment of potential treatment efficacy for protein therapeutics, so the book concludes with a chapter on establishing a clinical trials site. Written for the highly successful series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.
Authoritative and practical, serves as an ideal guide for researchers looking to develop new classes of drugs for treating disease, whether for rare or common disorders.
Zielgruppe
Professional/practitioner
Autoren/Hrsg.
Fachgebiete
- Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Medizinische Fachgebiete Pharmakologie, Toxikologie
- Technische Wissenschaften Verfahrenstechnik | Chemieingenieurwesen | Biotechnologie Biotechnologie
- Technische Wissenschaften Maschinenbau | Werkstoffkunde Technische Mechanik | Werkstoffkunde Materialwissenschaft: Biomaterialien, Nanomaterialien, Kohlenstoff
Weitere Infos & Material
Computational Design and Functional Evaluation of Novel Protein Drugs.- AI/In Silico Analysis of Protein-Protein Interactions.- Developing Stable Cell Lines with Doxycycline-Inducible Expression of Proteins for Functional Analysis and Protein-Protein Interactions.- RNA Interference Screening to Identify Novel Host-Pathogen Interactions.- Analyzing Virus-Host Protein-Protein Interactions via Co-Immunoprecipitation.- Biophysical Methods for the Characterization of Therapeutic Proteins.- Characterization of Serpin-Cofactor Interaction by Enzyme Kinetics, Affinity Chromatography, Differential Scanning Fluorimetry, and Isothermal Titration Calorimetry.- Production and Purification of the SerpinE2 PN-1 in a Procaryotic System.- Pigment Epithelium-Derived Factor and Its Receptor: Methods for Biochemical Analysis and Therapeutic Insights.- Cellular Models of Disease in the Evaluation of Therapeutic Targets.- A Method to Analyze Immune Cell Infiltration in the Tumor Microenvironment after Treatment with an Armed Oncolytic Virus.- Extraction of Silk Fibroin and Fabrication of Silk Biomaterials for Therapeutic Applications.- Transfection of Human Mature Adipocytes.- Purification of Small Extracellular Vesicles (sEVs) from Virus-infected Cells by Using Size Exclusion Chromatography and Yield-Enhancement via Activation of the Purinergic Receptor P2RX7.- Establishing Clinical Trials for Protein Therapeutics.
