Protein Architecture

1. Auflage 1999
ISBN: 978-0-8247-8236-8
Verlag: CRC Press

Buch, Englisch, 416 Seiten, Format (B × H): 347 mm x 218 mm, Gewicht: 658 g

Considers the design, structure and biological activity of ordered films comprised of proteins, polymers, amphiphile molecules and colloidal particles, and assesses the ability of protein/polyion multilayers to mimic living organelles. The book discusses how to reach predetermined locations with proteins and orient molecules while preserving their bioactivity.

Autoren/Hrsg.

Lvov/Mohwald

Fachgebiete

• Naturwissenschaften Biowissenschaften Proteinforschung

Inhaltsverzeichnis

Part 1 Protein adsorption on a solid surface: protein adsorption on solid/liquid interface. Part 2 Surface covalent immobilization of proteins: immobilization of protein monolayers on planar solid supports. Part 3 Protein monolayer formation at liquid interface and its transfer on solid substrate: quantitative detection of protein binding to the lipid membrane surface by using quartz crystal microbalance; ordering by capillary forces; Langmuir-Blodgett multilayers of proteins. Part 4 Electrostatic layer-by-layer assembly via alternate adsorption of proteins and polyions: electrostatic layer-by-layer assembly of proteins and polyions; sequential catalysis in organized multienzyme films; fabrication of immunoglobulin mono- and multilayers - their applications for immunosensors; immobilized enzyme multilayers -the way for assembly of microreactors and biosensors; ordered multilayer assemblies - albumin/heparin for biocompatible coating and monoclonal antibodies for optical immunosensors. Part 5 Multilayer assembly with biospecific interaction: biomaterials that communicate; antigen-antibody assembling of enzyme monomolecular layers and multilayers on electrodes. Part 6 Electrochemistry in ordered protein layers: electrochemistry and electroenzymology in layered films containing proteins. Part 7 Patterning of protein layers - two dimensional patterning of proteins.