Methods in Systems Bioinorganic Chemistry

Erscheinungsjahr 2026
ISBN: 978-0-443-52348-9
Verlag: Elsevier Science & Technology

Buch, Englisch, Format (B × H): 152 mm x 229 mm

Over the past half-century, the field of Bioinorganic chemistry, like so many branches of science, has been dominated by a reductionist approach to studying metal ions in biology. This incredibly successful strategy typically involved, and involves, isolating and characterizing individual metalloproteins. Although early researchers could imagine the complexity of the processes that involved these proteins, operating within cells, comprehending such processes on the systems level was simply out of reach. However, with the technical advances that have emerged over the past decade or so, considering such complex cellular-level interrelationships is becoming increasingly possible. This volume, entitled “Methods in Systems Bioinorganic Chemistry” is perhaps the first compilation of methods aimed at understanding high-level properties involving metals in biology founded on molecular or mechanistic level interactions. The importance of this cannot be overstated; establishing the roles of metal ions in human health and disease will undoubtedly require systems-level understandings. That being said, there is currently no established “tool kit” for studying bioinorganic systems, and so the methods included here span a diverse range of topics and approaches, from imaging to proteomics, from metals-in-mice to mathematical modeling. How these methods will eventually be integrated to advance systems-level insights is unfolding here and now. Enjoy!

Autoren/Hrsg.

Weitere Mitwirkende

Lindahl, Paul

Fachgebiete

• Naturwissenschaften Physik Angewandte Physik Biophysik
• Naturwissenschaften Biowissenschaften Molekularbiologie
• Naturwissenschaften Biowissenschaften Biochemie (nichtmedizinisch)

Inhaltsverzeichnis

1. Inductively Coupled Plasma Mass Spectrometry (ICP-MS)-based methods to quantify transition metals in cells and vertebrate models
Vishal Gohil, Natalie M. Garza and Fotoula T. Stroumpos
2. ICP-MS elemental analysis in tissue samples
Oleh Khalimonchuk and Javier Seravalli
3. An inductively-coupled-plasma triple quadrupole method with oxygen for the determination of essential elements and protein concentration
Anne Roberts and Blaine Roberts
4. Calculating metal speciation of proteins inside cells
Nigel J. Robinson and Arthur Glasfeld
5. Probing biological copper in the subfemtomolar regime
Christoph J. Fahrni
6. Using LC-ICP-MS chromatography to detect and characterize Labile Metal Pools
Paul Lindahl and Alexia C. Kreinbrink
7. Linking dynamic bioinorganic chemistry processes of toxic metals in the bloodstream to establish their exposure-response relationship in humans
Jürgen Gailer and Yemna Badar
8. Contextualizing X-ray fluorescent microscopy – high resolution X-rays meet subcellular morphology
Martina Ralle
9. Chemoproteomic profiling of transition metal binding sites in bacterial proteomes
David P. Giedroc, Maximillian K. Osterberg, Daniel W. Bak and Eranthie Weerapana
10. Heme Labeling by Proximity (HeLP) to Identify Hemoproteins
Amit R. Reddi
11. Profiling of metalloproteomes by METAL-TPP
Chu Wang
12. Using omics to understand the disorders of copper misbalance
Lorena Molina and Svetlana Lutsenko
13. Interrogating iron and manganese absorption, distribution, and excretion in mice using radioisotopes
Thomas Bartnikas, Milan Prajapati and Mitchell Knutson
14. Making sense of mammalian iron physiology through computational modeling
Pedro Mendes
15. Integrating Mechanistic and Data-Driven Approaches to Modeling Iron Transport in Epithelial Cells
Cristian Salgado
16. The Basic Pathways Approach to designing dynamical mathematical models of metal metabolism in growing cells
Paul Lindahl, Jay R. Walton and Justin Sun

Über Autor(innen)

Lindahl, Paul
Paul A. Lindahl has researched topics in the field of bioinorganic chemistry for nearly 50 years, and has seen its development and never-ending evolution. He obtained his PhD in chemistry from the Massachusetts Institute of Technology in 1985, and then moved to the University of Minnesota where he was a post-doctoral fellow in Eckard Münck’s lab. In 1988 he joined the faculty at Texas A&M University where he is currently Professor of Chemistry and of Biochemistry and Biophysics. He began his research in bioinorganic chemistry at the Illinois Institute of Technology as an undergraduate modeling the copper site in plastocyanin. After a year in graduate school at the University of California, Berkeley, he moved to MIT and joined the laboratory of William Orme-Johnson. There he studied the molybdenum-and-iron containing enzyme nitrogenase. During his post-doc, he used Mössbauer and EPR spectroscopies to study the nickel-and-iron containing bifunctional enzyme acetyl-coenzyme A synthase/carbon monoxide dehydrogenase, including how it interacts with a cobalt-and-iron containing protein. Thus, he has researched nearly all the transition metals used in nature. He continued to study this Ni-Fe-Co system for 2 decades at Texas A&M University, focusing on the mechanism of catalysis, including structural and spectroscopic aspects. In the late 1990’s he discovered that this enzyme was implicated in the iron-sulfur-world origin-of-life scenario proposed by Gunter Wächtershäuser, and this prompted a pivotal shift in his research towards metal metabolism in more complex cellular systems. In the past 2 decades, he has used the same biophysical methods that he used to study individual metalloenzymes to investigate metal metabolism in whole-cells and in subcellular structures such as mitochondria and cytosol. He also developed new bioanalytical methods by interfacing liquid chromatography with inductively-coupled plasma mass spectrometry to probe labile iron, copper, and zinc pools in various cellular systems, including their roles in cellular homeostatic regulation. He is currently using these tools to investigate the dysregulation of iron associated with hereditary hemochromatosis in mice (and by extension in humans). With the critical help of mathematicians, he has developed, and continues to develop, new approaches to quantitatively model iron and copper metabolism in eukaryotic cells using ordinary-differential equations. He looks forward to the day when these disparate approaches can be unified to clarify how biochemical processes are integrated within the human body to better understand metal-associated diseases.