Methods and Protocols
Buch, Englisch, Format (B × H): 178 mm x 254 mm
Reihe: Methods in Molecular Biology
ISBN: 978-1-0716-5690-7
Verlag: Springer Us
This volume details experimental and computational protocols that highlight modern quorum sensing research. Chapters guide readers through the molecular characterization of autoinducers and their receptors, individual and population level, and in the host and the environment. Written for the highly successful series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.
Authoritative and practical, provides well-established protocols used by both academics and industrials.
Zielgruppe
Professional/practitioner
Autoren/Hrsg.
Fachgebiete
Weitere Infos & Material
Mining QS-associated proteins using machine learning.- Predicting the acyl-homoserine lactone quorum sensing signal products of LuxI-type synthases using RefAHL.- Synthesis of Autoinducer-2 and its Analogues.- Characterizing novel AI-2 receptors using isothermal titration calorimetry.- Using activity-based probes to unravel quorum sensing mechanisms through chemical proteomics.- Methods for Discovering and Characterizing Archaeal Quorum Sensing Using Haloferax volcanii.- Quantifying phenotypic heterogeneity in Legionella quorum sensing using fluorescent reporters.- Single-cell approaches to study the role of quorum-sensing antiactivators in preventing self-sensing.- Dynamical Models to Study the Interplay between Multiple Quorum Sensing Systems.- Microscale Patterning for Multiscale Bacterial Signaling.- Developing and Characterizing standardized QS Systems for Gram-Negative Bacteria .- Designing and Implementing Quorum Sensing-Controlled CRISPRi Circuits for Dynamic Cell Programming.- Methods to map the QS response in fungi: the response of Ophiostoma piceae to farnesol.- The Hawaiian bobtail squid as a model organism for studies of quorum-signaling within host tissues.- Experimental Considerations for Studying Quorum Sensing in Marine Bacteria.- Measuring quorum sensing regulation of chitinase activity in Vibrio bacteria.- Experimental Approaches to Study AI-2 Chemotaxis In Vivo.- Sensing-Dependent Chemical Interactions in a Polymicrobial Cystic Fibrosis Model Using Untargeted Metabolomics.- Quorum-Sensing Control of Antiphage Defence Systems Detected by Double Agar Overlay Phage Plaque Assays.- Microbead-encapsulated bioreporter detection of QS signals in the environment.
