Mucins

Erscheinungsjahr 2026
ISBN: 978-0-443-43226-2
Verlag: Elsevier Science & Technology

Buch, Englisch, Format (B × H): 152 mm x 229 mm, Gewicht: 450 g

Mucins, Volume 732 serves as a valuable resource for researchers aiming to investigate mucin biology in greater depth, offering a wide range of experimental approaches and analytical strategies. Topics in this new release cover Enrichment/isolation, glycoproteomics, Mucinase cleavage characterization and usage in glycoproteomics, Fluorescent protein-based FRET substrates for activity screening of mucinases, Development of clinical lectin assays based on MS glycomic discovery, Enzymatic selective sulfation of synthetic mucins, cryoEM, Cell-based mucin arrays and glycoengineering, CRISPR screens to characterize and identify glycan-binding proteins, and much more.

Additional sections cover MD Simulations, Interactions with pathogenic bacteria, Interactions with microbiota, Covalent crosslinking of mucins, Liquid Glycan and Liquid Lectin Array-based exploration of musin recognition, Quantification of mucins, Mucins in IBD, Investigations of mucins and innate immunity, Generating synthetic mucin glycopeptides, and Microscopy and imaging.

Autoren/Hrsg.

Weitere Mitwirkende

Christianson, David

Allen, Karen N.

Malaker, Stacy

Fachgebiete

• Naturwissenschaften Biowissenschaften Molekularbiologie
• Naturwissenschaften Physik Angewandte Physik Biophysik
• Naturwissenschaften Biowissenschaften Biochemie (nichtmedizinisch)

Inhaltsverzeichnis

1. Enrichment/isolation, glycoproteomics
Stacy Malaker, Shane Finn and Sarah Lowery
2. Mucinase cleavage characterization and usage in glycoproteomics
Stacy Malaker, Alexandra Steigmeyer and Joann Chongsaritsinsuk
3. Fluorescent protein-based FRET substrates for activity screening of mucinases
Alisdair Boraston
4. Development of clinical lectin assays based on MS glycomic discovery
Niclas Karlsson, Ali Reza Afshari and Mary Solbego
5. Enzymatic selective sulfation of synthetic mucins
Jessica Kramer
6. cryoEM
Deborah Fass
7. Cell-based mucin arrays and glycoengineering
Yoshiki Narimatsu, Christian Bull, Henrik Clausen and Felix Goerdeler
8. CRISPR screens to characterize and identify glycan-binding proteins
Simon Wisnovsky
9. MD Simulations
Fiona Kearns
10. Interactions with pathogenic bacteria
Katharina Ribbeck
11. Interactions with microbiota
Eric Martens
12. Covalent crosslinking of mucins
Thomas Crouzier
13. Liquid Glycan and Liquid Lectin Array-based exploration of musin recognition
Ratmir Derda
14. Quantification of mucins
Sumaira Z. Hasnain
15. Mucins in IBD
Kirk Bergstrom
16. Investigatins mucins and innate immunity
Thaher Pelaseyed
17. Generating synthetic mucin glycopeptides
Peter Seeberger, Kabita Pradhan and Charlotte Völkel
18. Microscopy and imaging
Malin E.V. Johansson, George M H Birchenough, Elisabeth E L Nyström and Brendan Dolan

Über Autor(innen)

Allen, Karen N.
Dr. Karen N. Allen works at the Department of Chemistry of the Boston University, the Metcalf Center for Science and Engineering

Christianson, David
After completing studies for the A.B., A.M., and Ph.D. degrees in chemistry at Harvard University, David W. Christianson joined the faculty of the University of Pennsylvania, where he is currently the Roy and Diana Vagelos Professor in Chemistry and Chemical Biology. At Penn, Christianson’s research focuses on the structural and chemical biology of the zinc-dependent histone deacetylases as well as enzymes of terpene biosynthesis. His research accomplishments have been recognized by several awards, including the Pfizer Award in Enzyme Chemistry and the Repligen Award in Chemistry of Biological Processes from the American Chemical Society, a Guggenheim Fellowship, and the Elizabeth S. and Richard M. Cashin Fellowship from the Radcliffe Institute for Advanced Study at Harvard University. Christianson is also a dedicated classroom teacher, and his accomplishments in this regard have been recognized by the Lindback Award for Distinguished Teaching at Penn and a Rhodes Trust Inspirational Educator Award from Oxford University. Christianson has also held visiting professorships in the Department of Biochemistry at Cambridge University and the Department of Chemistry and Chemical Biology at Harvard University. Christianson has served with Prof. Anna Pyle as Co-Editor-in-Chief of Methods in Enzymology since 2015.