Protein Kinase Technologies

2012
ISBN: 978-1-61779-823-8
Verlag: Humana Press

Buch, Englisch, Band 68, 366 Seiten, Book w. online files / update, Format (B × H): 183 mm x 260 mm, Gewicht: 910 g

Reihe: Neuromethods

Divided into two convenient sections, Protein Kinase Technologies collects contributions from experts in the field examining recent methodologies and techniques generally applicable to protein kinase research as well as to individual protein kinases which require special attention in neuroscience.  These chapters will not only be practical instructions useful for readers' daily work in setting up and performing research but also thought-provoking and enjoyable reviews of recent advancements of individual protein kinases in neuroscience.  Written for the highly successful Neuromethods series, this work contains the kind of detailed description and implementation advice that is crucial for getting optimal results.

Authoritative and accessible, Protein Kinase Technologies seeks to foster cross-talk among investigators who study different protein kinases, and will also be beneficial for the entry of new investigators into this pivotal field.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Mukai, Hideyuki

Fachgebiete

• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Vorklinische Medizin: Grundlagenfächer Physiologie
• Naturwissenschaften Biowissenschaften Proteinforschung
• Naturwissenschaften Biowissenschaften Biowissenschaften Neurobiologie, Verhaltensbiologie
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Klinische und Innere Medizin Neurologie, Klinische Neurowissenschaft

Inhaltsverzeichnis

Visualization of Protein Kinase Activities in Living Cells- Phos-Tag Affinity Electrophoresis for Protein Kinase Profiling.- Large-Scale Protein Phosphorylation Analysis by Mass Spectrometry-Based Phosphoproteomics.- Ca/Calmodulin-Dependent Protein Kinase II (CaMKII).- CASK: A Specialized Neuronal Kinase.- Cyclin-Dependent Kinase 5 (Cdk5): Preparation and Measurement of Kinase Activity.- ErbB Membrane Tyrosine Kinase Receptors: Analyzing Migration in a Highly Complex Signaling System.- The Extracellular-Signal Regulated Kinase (ERK) Cascade in Neuronal Cell Signaling.- Glycogen Synthase Kinase-3 in Neurological Diseases.- Cortical Neurons Culture to Study c-Jun N-Terminal Kinase Signaling Pathway.- MuSK:  A Kinase Critical for the Formation and Maintenance of the Neuromuscular Junction.- Assays for Pten-Induced Novel Kinase 1 (PINK1) and Leucine-Rich Repeat Kinase 2 (LRRK2), Kinases Associated with Parkinson’s Disease.- Imaging PKA Activation Inside Neurons in Brain Slice Preparations.- Imaging Oscillations of Protein Kinase C Activity in Cells.- Diacylglycerol Kinase (DGK).- Trafficking of Trk Receptors.- Mammalian Target of Rapamycin.- Protein Kinase G (PKG):  Involvement in Promoting Neural Cell Survival, Proliferation, Synaptogenesis, and Synaptic Plasticity and the Use of New Ultrasensitive Capillary-Electrophoresis-Based Methodologies for Measuring PKG Expression and Molecular Actions.- Electrophysiological Technique for Analysis of Synaptic Function of PKN1 in Hippocampus.