Buch, Englisch, 499 Seiten, Format (B × H): 178 mm x 254 mm
Reihe: Methods in Molecular Biology
Methods and Protocols
Buch, Englisch, 499 Seiten, Format (B × H): 178 mm x 254 mm
Reihe: Methods in Molecular Biology
ISBN: 978-1-0716-5710-2
Verlag: Springer Us
This second edition provides new and updated chapters on methods for profiling and interpreting genomic alterations in tumors across both research and clinical settings. Chapters detail methods to interrogate DNA variation, RNA expression, and epigenetic changes using both next-generation sequencing and microarray techniques. Written in the highly successful
series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge,
aims to provide methods to generate and analyze data types to produce a molecular profile of tumors.
Zielgruppe
Professional/practitioner
Autoren/Hrsg.
Fachgebiete
Weitere Infos & Material
Amplicon-based targeted Next-Generation Sequencing of Formalin-Fixed Paraffin-Embedded tissue.- Hybridization-Based Library Preparation for Targeted Sequencing of Clinical Specimens.- Bioinformatics Basics for High-Throughput Hybridization-Based Targeted DNA Sequencing from Tumor Specimens: From Reads to Variants.- Annotation of variant data from high throughput DNA sequencing from tumor specimens: Filtering strategies to identify clinically relevant variants.- Clinical Validation of Comprehensive Solid Tumor Profiling by Next Generation Sequencing.- Long Read DNA Sequencing Using Oxford Nanopore Technology.- Whole Genome Single Nucleotide Polymorphism Microarray for Copy Number and Loss of Heterozygosity Analysis in Tumors.- Genome-Wide Copy Number Variation Detection using NGS: Data Analysis and Interpretation.- Structural and Copy Number Variant Detection Using Optical Genome Mapping.- Overview of Fusion Detection Strategies Using RNA Sequencing.- A computational strategy to uncover fusion genes in prostate cancer cell lines.- Deconstructing the Cancer Epigenome using Reduced Representation Bisulfite Sequencing (RRBS).- Tumor DNA Methylation Profiling: Methods, Insights, and Clinical Relevance.- Application of MeDIP-Sequencing (MeDIP – Seq) in Tumor Profiling.- Tumor-naïve genomic profiling of plasma-derived circulating tumor DNA using targeted next-generation sequencing.- Extraction and library preparation of cell-free microRNAs from peripheral blood.- A Gentle Introduction to Spatial Transcriptomic Analysis with 10X Visium Data.- Spatial Transcriptomics at Single Cell Resolution.- From Data to Discovery: Downstream Interpretation of High-Dimensional Omics Data for Precision Medicine.
