Introduction to Medical Virology

Theory and Practice in Tropical Countries
1. Auflage 2026
ISBN: 978-1-394-37638-4
Verlag: John Wiley & Sons Inc

Buch, Englisch, 544 Seiten, Format (B × H): 237 mm x 188 mm, Gewicht: 1043 g

ESSENTIAL KNOWLEDGE FOR CONFRONTING VIRAL DISEASES IN RESOURCE-LIMITED SETTINGS

Introduction to Medical Virology: Theory and Practice in Tropical Countries adapts contemporary advanced best practices in medical virology to tropical settings. Special emphasis is placed on viral infections that disproportionately affect tropical countries to ensure relevance to local disease burdens. It provides practical guidance on molecular methods, serology, point-of-care testing, and laboratory quality management, enabling readers to develop competence even in low-resource settings. The author brings extensive teaching and clinical expertise in viral pathogenesis, molecular biology, and pharmacology to deliver a comprehensive guide that bridges foundational virology with practical diagnostic and public health strategies.

Introduction to Medical Virology offers: - Clear explanations, step-by-step laboratory diagnostic procedures, case studies, and context-specific examples specifically designed for resource-limited settings, addressing infrastructure and equipment constraints
- Integration of clinical, epidemiological, and public health perspectives with real-world case studies that demonstrate application of virological principles to outbreak investigation and infection control
- Comprehensive coverage of vector dynamics, climate influences, and ecological factors that shape viral transmission patterns in tropical environments
- Accessible information on molecular biology and viral pathogenesis, balanced with hands-on laboratory protocols for specimen handling, testing, and biosafety
- An emphasis on arboviruses and tropical viral pathogens including dengue, chikungunya, Zika, hemorrhagic fevers, and emerging zoonotic diseases prevalent in tropical regions

Introduction to Medical Virology is designed for undergraduate and graduate students in medical laboratory sciences, microbiology, virology, public health, and biomedical sciences. The text is also intended for clinicians and public health professionals working in tropical regions. It provides the theoretical foundation and practical skills needed to diagnose, manage, and prevent viral infections effectively, strengthening healthcare capacity and contributing to global health security in the face of emerging and re-emerging viral threats.

Autoren/Hrsg.

Olasunkanmi, Oluwatayo Israel

Fachgebiete

• Naturwissenschaften Biowissenschaften Virologie
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Medizinische Fachgebiete Umweltmedizin, Arbeitsmedizin, Tropenmedizin, Sportmedizin Reisemedizin, Tropenmedizin
• Naturwissenschaften Biowissenschaften Mikrobiologie

Inhaltsverzeichnis

Forewords xxiii
Preface xxv
Acknowledgments xxvii
List of Abbreviations xxviii

1 General Features and Classification of Viruses of Medical Importance 1
1.1 Introduction 1
1.2 Basic Virus Structure 4
1.3 Classification of Viruses of Medical Importance 10
1.4 How Will Newly Identified Viruses Be Named? 27
1.5 Study Questions 28

2 Molecular Mechanisms of Virus Replication 35
2.1 Virus Attachment 35
2.2 Entry and Uncoating 42
2.3 Transcription of the Viral Genome 45
2.4 Translation and Posttranslational Modifications 54
2.5 Assembly 58
2.6 Maturation 62
2.7 Release or Exit 63
2.8 Study Questions 66

3 Principles of Cell Biology During Virus Infections 74
3.1 Cell Cycle Progression During Virus Replication 74
3.2 Cellular Response to Virus-Induced Stress 81
3.3 Cell Trafficking Machinery and Cytoskeleton During Viral Infection 94
3.4 Cell Transformation and Oncogenesis 6
3.5 Study Questions 99

4 Overview of Viral Genetics and Evolution in Tropical Virology 107
4.1 Overview of Viral Genome Structure 107
4.2 Genetic Basis of Emergence and Cross-Species Transmission in Tropical Virology 112
4.3 Overview of Virus Mutation 114
4.4 Genetic Recombination in Virus Infection 118
4.5 Molecular Evolution of Viruses 121
4.6 Future Directions in Viral Genetics and Evolution 124
4.7 MicroRNAs in Viral Genomes and Evolution 128
4.8 Study Questions 133

5 Immune Response to Viral Diseases 141
5.1 Innate Immunity 141
5.2 Adaptive Immune System 166
5.3 Virus Persistence and Immune Evasion 184
5.4 Virus-Induced Autoimmune Diseases 190
5.5 Study Question 191

6 Antivirals and Vaccines 204
6.1 Antivirals 204
6.2 Traditional Medicine 218
6.3 Screening for Antiviral Drugs 220
6.4 Antiviral Vaccination 224

7 Organization and Safety Management in Tropical Virology Laboratory 233
7.1 Organizational Management 233
7.2 Personal and Laboratory Safety and Precautions 242
7.3 Laboratory Waste Management 251
7.4 Sterilization, Decontamination, and Disinfection in a Virology Laboratory 254
7.5 Risk Assessment and Safety in the Virology Laboratory 259
7.6 Procedure for the Use of Biological Safety Cabinets (BSCs) 263
7.7 Storage of Biological Samples, Chemicals, Materials, and Apparatus 66
7.8 Study Questions 269

8 Methods of Cell Culture and Cell Line Maintenance: For Resource-Limited Settings 272
8.1 Types of Cell Culture 272
8.2 Facility, Material, and Methods for Cell Culture 283
8.3 Cell Passage, Subculture, or Splitting 295
8.4 Measuring Cell Growth and Growth Curves 301
8.5 Cryopreservation of Cells 303
8.6 Cell Culture Contamination and Infections 306
8.7 Sterility Testing for Cell Culture Contamination and Infections 312
8.8 Dealing with a Contaminated Cell Culture Room 315
8.9 Curing Irreplaceable Contaminated Cell Cultures 316
8.10 Study Questions 317

9 Methods of Laboratory Diagnosis of Viral Infection from Clinical Samples: Virus Cultivation, and Characterization 323
9.1 Selection, Collection, and Transportation of Clinical Samples 324
9.2 Methods for the Recovery of Virus Particles from Samples 341
9.3 Virus Isolation or Propagation 44
9.4 Molecular Diagnosis and Characterization of Viruses in Clinical Samples 382
9.5 Recording, Reporting, and Interpretation of Results in a Virology Laboratory 410
9.6 Study Questions 413

10 Public Health, Epidemiology, and Control of Viral Infections in Tropical Countries 419
10.1 Introduction to the Pathogenesis of Virus Infection 419
10.2 Geographical Distribution and Patterns of Virus Transmission 456
10.3 Modes of Transmission of Viral Infections 458
10.4 Surveillance and Reporting of Virus Infection 464
10.5 Public Health Strategies for Prevention and Control of Viral Diseases 467
10.6 "One Health" Approach to Controlling Viral Diseases 470
10.7 Challenges in the Control of Viral Infections 471
10.8 Global Health Initiatives 474
10.9 Viruses as Bioterrorism Agents: Implications for Biosecurity in Tropical Countries 476
10.10 Historical Outbreaks and Lessons Learned 478
10.11 Study Questions 480

Objectives 481
References 482
Index 490

Über Autor(innen)

OLUWATAYO ISRAEL OLASUNKANMI, PHD, is an American Society for Clinical Pathologists (ASCPi)-certified Medical Laboratory Scientist with extensive experience in the United States, specializing in viral pathogenesis and diagnostic virology. He has also worked as a Research Associate at the University of Nebraska Medical Center, USA, and First Affiliated Hospital of Chongqing Medical University, China. As a registered member of the Medical Laboratory Science Council of Nigeria, he has led viral diagnostic services, epidemiological surveillance activities, and disease eradication efforts in both tertiary and rural hospitals across the country. His work focuses on Global health and understanding viral infections in tropical settings, developing practical approaches to viral disease management in resource-limited settings.