Investigation of Nanoscopic Dynamics and Potentials by Interferometric Scattering Microscopy

Softcover Nachdruck of the original 1. Auflage 2018
ISBN: 978-3-030-08368-7
Verlag: Springer International Publishing

Buch, Englisch, 142 Seiten, Previously published in hardcover, Format (B × H): 155 mm x 235 mm, Gewicht: 283 g

Reihe: Springer Theses

This thesis offers a unique guide to the development and application of ultrasensitive optical microscopy based on light scattering. Divided into eight chapters, it covers an impressive range of scientific fields, from basic optical physics to molecular biology and synthetic organic chemistry. Especially the detailed information provided on how to design, build and implement an interferometric scattering microscope, as well as the descriptions of all instrumentation, hardware interfacing and image processing necessary to achieve the highest levels of performance, will be of interest to researchers now entering the field.

Zielgruppe

Research

Autoren/Hrsg.

Ortega Arroyo, Jaime

Fachgebiete

• Naturwissenschaften Chemie Physikalische Chemie
• Naturwissenschaften Physik Quantenphysik Atom- und Molekülphysik
• Naturwissenschaften Chemie Organische Chemie
• Naturwissenschaften Biowissenschaften Zellbiologie

Inhaltsverzeichnis

Introduction.- Non-fluorescent single-molecule approaches to optical microscopy.- Experimental Methods.- Anomalous diffusion due to interleaflet coupling and molecular pinning.- Structural dynamics of myiosin 5a.- All optical label-free detection, imaging and tracking of single proteins.- Single-molecule chemical dynamics: direct observation of physical autocatalysis.- Outlook.

Über Autor(innen)

Jaime Ortega-Arroyo is currently a postdoctoral researcher in Romain Quidant’s plasmon nano-optics group at the ICFO (Institute of Photonic Sciences) in Barcelona, Spain. His current research focuses on combining ultrasensitive light microscopy with plasmon-assisted optofluidics. He received his BSc in Chemistry and Mathematics from the University of British Columbia, Vancouver, Canada in 2010, and his PhD in Physical and Theoretical Chemistry from the University of Oxford, UK in 2015, under the guidance of Phillip Kukura.