Mitochondrial Regulation

Methods and Protocols
2. Auflage 2021
ISBN: 978-1-0716-1435-8
Verlag: Springer

Buch, Englisch, 318 Seiten, Format (B × H): 178 mm x 254 mm, Gewicht: 626 g

Reihe: Methods in Molecular Biology

This fully updated edition explores the different pathways that converge into the regulation of mitochondrial function. The book integrates mitochondria with other cellular components, discussing the dynamic properties of mitochondria with an emphasis on how these processes respond to signaling events and how they affect cellular metabolism. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and up-to-date, Mitochondrial Regulation: Methods and Protocols, Second Edition is an ideal guide for advanced undergraduates, graduates, postgraduates, and beginning researchers in the areas of molecular and cellular biology, biochemistry, and bioenergetics.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Palmeira, Carlos M.

Rolo, Anabela P.

Fachgebiete

• Naturwissenschaften Biowissenschaften Angewandte Biologie Biophysik
• Naturwissenschaften Physik Angewandte Physik Biophysik
• Naturwissenschaften Biowissenschaften Zellbiologie

Inhaltsverzeichnis

1. Isolation of Mitochondria from Liver and Extraction of Total RNA and Protein: Analyses of miRNA and Protein Expression

            André L. Simão, Pedro M. Borralho, Clifford J. Steer, Rui E. Castro, and Cecília M.P. Rodrigues

2. Determination of Oxidative Phosphorylation Complexes Activities

            João S. Teodoro, Ivo F. Machado, Carlos M. Palmeira, and Anabela P. Rolo

3. BN-PAGE-Based Approach to Study Thyroid Hormones and Mitochondrial Function

            Elena Silvestri, Assunta Lombardi, Federica Cioffi, and Fernando Goglia

4. Monitoring Mitochondrial Function in Mouse Embryonic Stem Cells (mESCs)

            Bibiana Correia, Maria Inês Sousa, Ana F. Branco, and João Ramalho-Santos

5. Mitochondrial Functional Assessment in Mammalian Gametes Using Fluorescent Probes

            Renata S. Tavares, Sara-Escada-Rebelo, Maria M. Soares, Andreia F. Silva, Teresa Almeida-Santos, Sandra Amaral, Ana Paula Sousa, and João Ramalho-Santos

6. Update on the Histoenzymatic Methods for Visualization of the Activity of Individual Mitochondrial Respiratory Chain Complexes in the Human Frozen Sections

            Mariusz R. Wieckowski, Maciej Pronicki, and Agnieszka Karkucinska-Wieckowska

7. An Update on Isolation of Functional Mitochondria from Cell for Bioenergetics Studies

            Magdalena Lebiedzinska-Arciszewska, Lech Wojtczak, and Mariusz R. Wieckowski

8. PCR-Based Determination of Mitochondrial DNA Copy Number in Multiple Species

            Tess C. Leuthner, Jessica H. Hartman, Ian Ryde, and Joel N. Meyer

9. Methods to Monitor Mitophagy and Mitochondrial Quality: Implications in Cancer, Neurodegeneration, and Cardiovascular Diseases

            Simone Patergnani, Massimo Bonora, Esmaa Bouhamida, Alberto Danese, Saverio Marchi, Giampaolo Morciano, Maurizio Previati, Gaia Pedriali, Alessandro Rimessi, Gabriele Anania, Carlotta Giorgi, and Paolo Pinton

10. Analysis of Pro-Apoptotic Protein Trafficking to and from Mitochondria

            Ignacio Vega-Naredo, Gabriela Oliveira, Teresa Cunha-Oliveira, Teresa Serafim, Vilma A. Sardao, and Paulo J. Oliveira

11. Exploring Liver Mitochondrial Function by C-Stable Isotope Breath Tests: Implications in Clinical Biochemistry

            Emilio Molina-Molina, Harshitha Shanmugam, Domenica Di Palo, Ignazio Grattagliano, and Piero Portincasa

12. Protocols for Mitochondria as the Target of Pharmacological Therapy in the Context of Nonalcoholic Fatty Liver Disease (NAFLD)

            Ignazio Grattagliano, Agostino Di Ciaula, Jacek Baj, Emilio Molina-Molina, Harshitha Shanmugam, Gabriella Garruti, David Q.-H. Wang, and Piero Portincasa

13. The Use of Reactive Oxygen Species Production by Succinate-Driven Reverse Electron Flow as an Index of Complex 1 Activity in Isolated Brown Adipose Tissue Mitochondria

            Andrea Dlasková, Kieran J. Clarke, Mary F. Rooney, and Richard K. Porter

14. Mitochondrial Regulation Assessment by C-NMR Isotopomer Analysis

            Francisco X. Carvalho, Bárbara Guerra-Carvalho, Ivana Jarak, and Rui A. Carvalho

15. Assays for Determination of Cellular and Mitochondrial NAD and NADH Content

            Yue Yang and Anthony A. Sauve

16. Following the Dynamism of the Mitochondrial Network in T Cells

            Arianna Di Daniele, Luca Simula, and Silvia Campello

17. Measuring PGC-1a and Its Acetylation Status in Mouse Primary Myotubes

            João A. Amorim and David A. Sinclair