Animal Cell Biotechnology

Methods and Protocols
2. Auflage 2007
ISBN: 978-1-58829-660-3
Verlag: Humana Press

Buch, Englisch, Band 24, 512 Seiten, HC runder Rücken kaschiert, Format (B × H): 160 mm x 241 mm, Gewicht: 1054 g

Reihe: Methods in Biotechnology

Animal Cell Biotechnology: Methods and Protocols, Second Edition constitutes a comprehensive manual of state-of-the-art and new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and for optimizing critical parameters for cell culture considering the whole cascade from lab to final production. The volume is divided into five parts that reflect the processes required for different stages of production. In Part I, basic techniques for establishment of production cell lines are addressed, especially transduction techniques, cells for gene therapy and antibody production. Part II addresses basic cultivation techniques, such as microcarrier culture and encapsulation.

Part III covers cell characterization and analysis, including flow cytometric applications, NMR-based techniques, and biochemical and cytometric techniques. Part IV details cultivation techniques, such as disposable bioreactors, hollow fiber cell culture, fixed bed reactors, and configuration of bioreactors. Part V covers downstream techniques such as membrane filtration techniques, while Part VI describes special applications, including retroviral vectors.

Animal Cell Biotechnology: Methods and Protocols, Second Edition provides a compendium of techniques for scientists in industrial and research laboratories that use mammalian cells for biotechnology purposes.

Zielgruppe

Research

Autoren/Hrsg.

Pörtner, Ralf

Fachgebiete

• Technische Wissenschaften Verfahrenstechnik | Chemieingenieurwesen | Biotechnologie Biotechnologie
• Naturwissenschaften Biowissenschaften Zellbiologie
• Naturwissenschaften Biowissenschaften Proteinforschung
• Naturwissenschaften Biowissenschaften Biowissenschaften DNA und Transgene Organismen

Inhaltsverzeichnis

Basic Techniques for Establishing Production Cell Lines.- Transduction Technologies.- Cells for Gene Therapy and Vector Production.- Technology and Production of Murine Monoclonal and Recombinant Antibodies and Antibody Fragments.- DNA Fingerprinting and Characterization of Animal Cell Lines.- Basic Cultivation Techniques.- Microcarrier Cell Culture Technology.- Cell Encapsulation.- Tools for High-Throughput Medium and Process Optimization.- Cell Characterization and Analysis.- Cell Counting and Viability Measurements.- Monitoring of Growth, Physiology, and Productivity of Animal Cells by Flow Cytometry.- Nuclear Magnetic Resonance Methods for Monitoring Cell Growth and Metabolism in Intensive Bioreactors.- Methods for Off-Line Analysis of Nutrients and Products in Mammalian Cell Culture.- Application of Stoichiometric and Kinetic Analyses to Characterize Cell Growth and Product Formation.- Measurement of Apoptosis in Cell Culture.- Metabolic Flux Estimation in Mammalian Cell Cultures.- Special Cultivation Techniques.- Disposable Bioreactors for Inoculum Production and Protein Expression.- Hollow Fiber Cell Culture.- Cultivation of Mammalian Cells in Fixed-Bed Reactors.- Configuration of Bioreactors.- Downstream Techniques.- Membrane Filtration in Animal Cell Culture.- Chromatographic Techniques in the Downstream Processing of (Recombinant) Proteins.- Special Applications.- Vaccine Production.- Retrovirus Production and Characterization.- Insect Cell Cultivation and Generation of Recombinant Baculovirus Particles for Recombinant Protein Production.