Buch, Englisch, 272 Seiten, Format (B × H): 178 mm x 254 mm
Reihe: Methods in Molecular Biology
Methods and Protocols
Buch, Englisch, 272 Seiten, Format (B × H): 178 mm x 254 mm
Reihe: Methods in Molecular Biology
ISBN: 978-1-0716-5666-2
Verlag: Springer Us
This book looks at the latest advancements in prime editing. The chapters in this book cover pegRNA design and assembly; optimizations for prime editing in mammals; plants and yeast; approaches for quantification of on-target and off-target events; and viral delivery. Written in the highly successful series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Thorough and practical, is a valuable resource that provides researchers with detailed and practical methodology for implementing prime editing across a wide range of biological systems.
Zielgruppe
Professional/practitioner
Autoren/Hrsg.
Fachgebiete
Weitere Infos & Material
Prime Editing: Mechanism and Developments.- pegRNA Online Tool Design and Experimental Assembly: Exploring the Practical Use of PE-Designer for Prime Editing.- Design of Highly Efficient pegRNAs with Minimized Off-Target Effects Using DeepPrime.- Using Prime Editing Guide Generator (PEGG) for High-Throughput Generation of Prime Editing Sensor Libraries.- Rapid Cell-Free Preparation of Circular Vector DNA for pegRNA Expression in Prime Editing.- Construction of the p2PE3 Platform Based on RNA-Pol-II-Driven Engineered pegRNA.- Protocol for Prime Editing with Engineered PAM-Flexible Cas9 Variants.- Improvement of Genome Editing Efficiency in Mammalian Cells by A Refined Uni-Vector Prime Editing System.- Optimization and Evaluation of Prime Editing Activity in Human iPSCs.- All-in-One Prime Editing System for Efficient Generation and Isolation of Single Edited hiPSCs Clones.- Large Insertion Using Template-Jumping Prime Editing.- PE-STOP for Precise Installation/Reversion of Nonsense Mutation.- Quantification of Prime Editor-Corrected Frameshift Mutations Using Bioluminescence Resonance Energy Transfer (BRET).- Functional Analysis of Genetic Variants Using High-Throughput Prime Editing Screening.- Digital PCR Workflow for Prime Editing Quantification and Analysis.- TAPE-seq: A Cell-Based Method for Genome-Wide Off-Target Prediction in Prime Editing.- Protocol for Prime Editing in Human Erythroid Cell Lines Using Lentiviral Delivery.- Plant Prime Editing: Design, Assembly, and Application.- Prime Editing-Mediated Genome Modification in
