Buch, Englisch, 130 Seiten, Format (B × H): 178 mm x 254 mm
Reihe: Springer Protocols Handbooks
Buch, Englisch, 130 Seiten, Format (B × H): 178 mm x 254 mm
Reihe: Springer Protocols Handbooks
ISBN: 978-1-0716-5837-6
Verlag: Springer Us
This second edition details state-of-the-art computational and experimental protocols for the characterization of functional peptide molecules. Chapters cover ‘in silico’ peptide design and optimization using molecular dynamics simulations, molecular docking, and electrostatic potential modeling. The book further guides readers through solid-phase peptide synthesis alongside chromatographic and mass spectrometric characterization techniques. Importantly, it presents specialized methods for biophysical and functional characterization across key applications, including peptide-based drug delivery, nano-assembly, bionanocatalysis, protein aggregation, antibacterial ultrastructural analysis, and environmental bioremediation.
Authoritative and cutting-edge, serves as a vital resource for emerging and established researchers in peptide chemistry, biophysics, and bionanotechnology.
Zielgruppe
Professional/practitioner
Autoren/Hrsg.
Fachgebiete
Weitere Infos & Material
1. De novo peptide design tools.- 2. Molecular docking.- 3. Molecular Dynamics Simulations.- 4. Conformational analysis in MD Simulations of Peptides.- 5. Modelling Electrostatic Potential around Peptides.- Part II: SYNTHESIS AND CONFORMATIONAL CHARACTERIZATION.- 6. Peptide Synthesis.- 7. Peptide Purification by HPLC.- 8. Mass Spectrometry.- 9. Circular Dichroism (CD) Spectroscopy.- 10. Peptide secondary structure from Fourier Transform Infrared (FTIR) Spectroscopy.- 11. Estimation of Protein and Peptide Concentration by UV visible spectroscopy.- 12. Cell Culture for Cell Penetrating Peptides.- 13. Comparative uptake of peptides by Flow cytometry.- 14. Cellular uptake of peptides by Confocal Laser Scanning Microscopy.- 15. Fluorescence spectroscopic methods for Peptide Characterization.- 16 Cellular uptake using Fluorescence imaging.- 17. Penetration in 3D tumorosphere.- 18. TMRM Cytotoxicity Assay.- 19. Cell death by apoptosis.- 20. Serum compatibility of peptides.- 21. Antimicrobial assays with peptides.- 22. Antimicrobial Assay with peptide based hydrogels.- 23. Field Emission Scanning Electron Microscopy (FE-SEM) for antimicrobial activity.- 24. Hemolytic activity.- 25. Rheology of Peptide based Hydrogels.- 26. Estimation of Aggregation Kinetics.- 27. Assessment of amyloid morphology using electron microscopy.- 28. Size distribution of structures using Dynamic light scattering (DLS).- 29. Estimation neurotoxicity in neuronal cells.- Part VI: BIONANO CATALYSIS.- 30. Catalytic efficiency of designed peptides.- 31. Bioremediation using Atomic Emission Spectroscopy.
