Stepwise Culture of Human Adult Stem Cells

1. Auflage 2024
ISBN: 978-87-7022-854-1
Verlag: River Publishers

Buch, Englisch, 176 Seiten, Format (B × H): 161 mm x 240 mm, Gewicht: 435 g

Reihe: River Publishers Series in Biotechnology and Medical Research

This volume on stepwise techniques to culture adult human stem cells begins with the basic methods to culture adult stem cells followed by related methods to supplement stem cell culture. This and future volumes of techniques will allow laboratories across scientific entities – academic and commercial – to adapt the described methods for similar methods with cells from other species. The standardized techniques will allow across the board interpretation of other technologies such as Omics studies. These methods can be a starting platform for culturing stem cells even in laboratories that might not be familiar with the techniques.

Scientific and early clinical trials strongly favor the development of translational studies with adult stem cells. A major impediment to progress in the field of regenerative medicine is a lack of standardized methods to culture specific stem cells. Such documented methods would allow for scientific rigor and reproducibility to enhance efficient and safe translation of stem cells to patients. This book fills that gap.

Zielgruppe

Postgraduate and Professional Practice & Development

Autoren/Hrsg.

Rameshwar, Pranela

Fachgebiete

• Naturwissenschaften Biowissenschaften Biowissenschaften
• Naturwissenschaften Physik Mechanik Energie

Inhaltsverzeichnis

Introduction: Core Techniques in Stem Cell Culture

1. Isolation of Hematopoietic Stem Cells

2. Isolation Protocol for CD133+ and CD34+ Very Small Embryonic Like Cells (VSEL) from Human Umbilical Cord Blood

3. Isolation and Expansion of Mesenchymal Stem Cells from the Adult Bone Marrow Aspirate

4. Manual Isolation and Expansion of Adipose Derived Stem Cells from Adipose Tissue

5. Isolating Compact Bone-derived Mesenchymal Stem Cells from Rodent and Rabbit Femurs

6. Isolating Dental Pulp Stem Cells

7. A Murine System for Somatic Cellular Reprogramming into Induced Pluripotent Stem Cells

8. Isolation and Expansion Of Placenta Stem Cells (P-MSCs)

9. Expanding Human Mesenchymal Stem Cells from the Umbilical Cord

10. Laboratory Isolation of Leukemia Cancer Stem Cell

11. Isolation and Characterization of Cancer Stem cells from Glioblastoma Multiforme and Breast Cancer Cell Lines

12. Bone Marrow Aspirate for Isolation of Mesenchymal Stem Cells

13. Culturing Human Bone Marrow Stromal Cells

14. Isolating Mononuclear Cells by Ficoll-Hypaque Density Gradient

15. Phenotypic and Multipotent Characterization of Bone Marrow-derived Mesenchymal Stem Cells

16. Cryopreservation of Relatively Small Number of Stem Cells

17. Cryopreservation of Mobilized Peripheral Blood Stem Cells

18. Manual Cell Count

Über Autor(innen)

Pranela Rameshwar is a tenured professor of medicine, Division of Hematology and Oncology at Rutgers New Jersey Medical School, Rutgers. She received her B.Sc. degree in medical microbiology from the University of Wisconsin at Madison and Ph.D. in biology from Rutgers University, New Jersey. She performed postdoctoral studies in hematopoiesis at New Jersey Medical School. Dr. Rameshwar’s research interest in stem cell biology began with her doctoral thesis and continues with research on breast cancer dormancy, neural regulation of hematopoiesis, and clinical application of adult human mesenchymal stem cells. Her research continues to be funded by federal, state, and other agencies. Dr. Rameshwar has edited books and authored >200 publications, which include original articles, reviews, editorials and book chapters. Dr. Rameshwar initiated a certificate program in stem cell biology and directs four graduate level courses in stem cell biology.