Crop Genome Editing Using Crispr/Cas9

Theory and Practice
Erscheinungsjahr 2019
ISBN: 978-0-12-816672-7
Verlag: Elsevier Science

Buch, Englisch, 250 Seiten, Format (B × H): 191 mm x 235 mm, Gewicht: 504 g

Crop Genome Editing Using CRISPR/Cas9: Theory and Practice is a highly useful reference for implementing genome engineering technologies, particularly CRISPR related projects in agricultural crops and other plants. This book provides an introduction to CRISPR's basic science and applied aspects, along with detailed protocols. It presents a detailed workflow, beginning with genome sequence retrieval and then mutation analysis in genome edited events using sequencing tools. The book helps those in the field methodically plan, design and conduct experiments. This practical guide will dramatically help researchers in accelerating conventional plant breeding programs.

Zielgruppe

Graduate students, Postdoctoral fellows, scientists, professors in plant and crop science.

Autoren/Hrsg.

Ravindhran, Ramalingam

Ganesan, Govindan

Vinoth, A.

Fachgebiete

• Naturwissenschaften Biowissenschaften Biowissenschaften Genetik und Genomik (nichtmedizinisch)

Inhaltsverzeichnis

1. Introduction to genome editing2. Crop improvement using CRISPR/Cas93. Designing of sgRNA4. Tools and Resources for the construction of CRISPR vectors5. Cloning of CRISPR constructs6. Protoplast Isolation from plants7. Delivery of CRISPR constructs into protoplasts and mutation analysis8. Agrobacterium mediated transformation of CRISPR constructs for the generation of stable mutant plants9. Recent Advancements in CRISPR/Cas9 genome editing in plants

Über Autor(innen)

Vinoth, A.
Assistant Professor, Department of Botany, St. Xavier's College, Tamil Nadu, India

Ganesan, Govindan
Research Assistant Professor, Department of Genetic Engineering, SRM University, Kattankulathur, India

Ravindhran, Ramalingam
Professor and Head, Department of Plant Biology and Biotechnology, Loyola College, Chennai, India