Bioanalytics for Beginners

2012
ISBN: 978-1-4614-0922-9
Verlag: Springer

Buch, Englisch, 415 Seiten, Format (B × H): 155 mm x 260 mm

Bioanalytics for Beginners is a translation of the wildly successful German text, Bioanalytik für Einsteiger. This volume goes far beyond simply reviewing modern and sophisticated biochemical and molecular biological laboratory methods. Instructional design elements complement the vividly written text, through the use of charts exploring complex bio-processes, full-color three-dimensional graphics of important molecular structures, and boxes highlighting historical aspects behind the scientists whose ideas and experiments have led us to modern bioanalytic research.

With contributions by leading experts in research and industry, Dr. Renneberg's Bioanalytics for Beginners provides a thorough introduction to bioanalytics, suitable for both the classroom and everyday use. Academic researchers and students will gain a comprehensive understanding of bioanalytics in a variety of contexts, such as its application to drugs and HIV tests, blood sugar determination in diabetes, the measurement of physical fitness, and many others. This book will excite its readers and and arouse the curiosity for more!

Zielgruppe

Upper undergraduate

Autoren/Hrsg.

Renneberg, Reinhard

Fachgebiete

• Naturwissenschaften Biowissenschaften Molekularbiologie
• Naturwissenschaften Chemie Organische Chemie Biochemie
• Technische Wissenschaften Verfahrenstechnik | Chemieingenieurwesen | Biotechnologie Biotechnologie Biotechnologie: Mikrotechnologie, Nanobiotechnologie
• Naturwissenschaften Biowissenschaften Biochemie (nichtmedizinisch)
• Technische Wissenschaften Sonstige Technologien | Angewandte Technik Medizintechnik, Biomedizintechnik
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Medizin, Gesundheitswesen Medizintechnik, Biomedizintechnik, Medizinische Werkstoffe
• Technische Wissenschaften Verfahrenstechnik | Chemieingenieurwesen | Biotechnologie Biotechnologie Medizinische Biotechnologie

Inhaltsverzeichnis

PREFACE
CHAPTER 1 The Nanoru - The incredible story of its isolation, purification, and characterization 1.1 The Site 1.2 Rearing and pure culture 1.3 Biomass production 1.4 Activity Test 1.5 Gel and ion exchange chromatography 1.6 Affinity chromatography 1.7 Isoelectric focusing 1.8 Gel electrophoresis 1.9 Mass and sequence analysis 1.10 How the Gen was "fished" 1.11 X-ray structure and NMR 1.12 The Sensation: The Nanoru - sudden clarity 1.13 How do we continue with the Nanoru?
CHAPTER 2 Biomolecules in tests - Instrumental Bioanalytics 2.1 More and more machines for ever-smaller particles 2.2 Protein separation 2.3 Gel filtration chromatography separates proteins by size 2.4 Ion exchange chromatography separates proteins after their charge 2.5 Molecular Ying and Yang: affinity chromatography 2.6 High-performance liquid chromatography (HPLC) 2.7 What occurs with purification? Electrophoresis analyses protein mixtures qualitatively 2.8 Isoelectric focusing separates proteins according to their neutral points 2.9 Capillary electrophoresis combines high selectivity with brief periods of separation 2.10 Antibody probes identify proteins 2.11 The instrumental exploration of protein structure 2.12 Edman - Sequencing deciphered the primary structure of a protein 2.13 Mass spectrometry determined exact protein and peptide primary structure 2.14 X-ray structure deciphered protein conformation 2.15 The nuclear resonance spectroscopy (NMR) studied proteins in solution

CHAPTER 3 Biocatalysis - enzymes and enzyme tests 3.1 Enzymes: highly specific and efficient molecular machines 3.2 Chicken or egg? Ribozymes are also biocatalysts 3.3 How enzymes recognize substrates 3.4 How enzymes are named and classified 3.5 Key-lock or hand glove? 3.6 Coenzymes are transformed like substrates 3.7 Enzyme kinetics: how enzyme reactions are in the time progress 3.8 Unit and Katal: the measurement of enzyme activity 3.9 Getting started: optical enzyme tests 3:10 Dry chemistry: from the litmus test paper for glucose-testing 3.11 Inhibition of enzyme reactions 3.12 Bird dead or…: the exact measurement of enzyme inhibitors 3.13 Isoenzymes 3.14 Enzyme-activity tests
CHAPTER 4 Bio-affinity I: antibodies and immune tests 75 4.1 Is War vaccination successful? The ring test 4.2 How antigens and antibodies react with Haptene 4.3 Blood is a very special juice: determination of blood groups 4.4 Soluble + soluble = insoluble: immune precipitation 4.5 Diffusion combined with electrophoresis: immune electrophoresis 4.6 Useful protein blurs: Western blotting 4.7 Nephelometry: to fish in the dark with success 4.8 Immuno assays: "The best [perfect] is the enemy of the good" 4.9 Thyroid tests with the Radio immuno assay 4.10 Immunology with the power of enzymes: ELISA 4.11 Indirect ELISA: detection of antibodies against HIV and the Dot test 4.12 Rapid immuno-tests: a baby – yes or no? 4.13 The rapid detection of a death angel: HIV tests 4.14 Quick help with heart attacks 4.15 A worldwide trend: Point of care (POC) tests 4.16 Drugs and their abuse 4.17 Immunological drug tests 4.18 How to evaluate a laboratory test: sample HIV testing 4.19 How to test tests: ROC-curves

CHAPTER 5 Bio-affinity II: biological receptors - nature as unsurpassed Bioanalysis lab worker 5.1 The fantastic dog nose: A million-times better than ours! 5.2 Our human meaning 5.3 Smell: olfactory detection 5.4 How does a receptor work? 5.5 Electronic nose: Combined polymers versus real receptors 5.6 Taste: gustatory detection 5.7 Visual det

Über Autor(innen)

Renneberg, Reinhard
Reinhard Renneberg has been a Professor of Analytic Biotechnology at the Hong Kong University of Science and Technology (www.ust.hk) since 1955. He is the author of the extremely successful and much-lauded volume Biotechnology for Beginners, of which over 5,300 copies have been sold in 18 months. He has also written three other books on biotechnology, including Katzenklon. He is a co-author of the Roempp Biotechnology Dictionary, has written four monographs and 250 publications and holds 20 patents. In addition, he is involved in two biotechnology companies in Germany and China.
Oliver Kayser teaches at the University of Groningen. He has written a number of books on pharmaceutical biotechnology, including Essentials of Pharmaceutical Biotechnology, Pharmaceutical Biotechnology, and Medicinal Plant Biotechnology.
Darja Süßbier is a scientific illustrator who has contributed to numerous volumes in the past. With her appealing four-color layout and numerous bespoke graphics for the book, she has given this volume an immeasurably distinctive touch.

Reinhard Renneberg has been a Professor of Analytic Biotechnology at the Hong Kong University of Science and Technology (www.ust.hk) since 1955. He is the author of the extremely successful and much-lauded volume Biotechnology for Beginners, of which over 5,300 copies have been sold in 18 months. He has also written three other books on biotechnology, including Katzenklon. He is a co-author of the Roempp Biotechnology Dictionary, has written four monographs and 250 publications and holds 20 patents. In addition, he is involved in two biotechnology companies in Germany and China.