Planarian Regeneration

Methods and Protocols
1. Auflage 2018
ISBN: 978-1-4939-7800-7
Verlag: Springer

Buch, Englisch, Band 1774, 573 Seiten, Book w. online files / update, Format (B × H): 183 mm x 260 mm, Gewicht: 1421 g

Reihe: Methods in Molecular Biology

This volume explores the various facets of planaria as a biomedical model system and discusses techniques used to study the fascinating biology of these animals. The chapters in this book are divided into two parts: Part One looks at the biodiversity of planarian species, the molecular orchestration of regeneration, ecology of planarians in their natural habitats and their history as lab models. Part Two talks about experimental protocols for studying planarians, ranging from the establishment of a planarian research colony, to RNA and DNA extraction techniques, all the way to single stem cell transplantations or metabolomics analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.??

Comprehensive and cutting-edge, Planarian Regeneration: Methods and Protocols is a valuable resource for both newcomers to the field and experts within established planarian laboratories.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Rink, Jochen C.

Fachgebiete

• Naturwissenschaften Biowissenschaften Biowissenschaften Ökologie
• Naturwissenschaften Biowissenschaften Zellbiologie
• Naturwissenschaften Biowissenschaften Tierkunde / Zoologie Wirbellose (Invertebrata)
• Naturwissenschaften Biowissenschaften Tierkunde / Zoologie Tierökologie

Inhaltsverzeichnis

Preface…Table of Contents…Contributing Authors…
1. Planarian Diversity and PhylogenyRonald Sluys and Marta Riutort
2. Stem Cells, Patterning, and Regeneration in Planarians-Self-Organization at the Organismal ScaleJochen Rink
3. The Ecology of Freshwater PlanariansMiquel Vila-Farré and Jochen C. Rink
4. Planarians and the History of Animal Regeneration: Paradigm Shifts and Key Concepts in BiologySarah A. Elliott and Alejandro Sánchez Alvarado
5. Culturing Planarians in the LaboratoryM. Shane Merryman, Alejandro Sánchez Alvarado and James C. Jenkins
6. Total RNA Isolation from Planarian TissuesShang-Yun Liu and Jochen C. Rink
7. Small- and Large-Scale High Molecular Weight Genomic DNA Extraction from PlanariansMarkus A. Grohme, Miquel Vila-Farré, and Jochen C. Rink
8. Planarian High Molecular Weight DNA Isolation by SpoolingShasha Zhang and Alejandro Sánchez Alvarado
9. Basic Histological Techniques for PlanariansLeigh Winsor and Ronald Sluys
10. Fixation, Processing, and Immunofluorescent Labeling of Whole Mount PlanariansDavid J. Forsthoefel, Kelly G. Ross, Phillip A. Newmark, and Ricardo M. Zayas
11. Immunohistochemistry on Paraffin-Embedded Planarian Tissue SectionsTeresa Adell, Sara Barberán, Miquel Sureda-Gómez, María Almuedo-Castillo, Nidia de Sousa, and Francesc Cebriá
12. Whole-Mount In Situ Hybridization of PlanariansRyan S. King and Phillip A. Newmark
13. RNA In Situ Hybridization on Planarian Paraffin SectionsJordi Solana
14. General Principles of Planarian Embryogenesis and Its Analysis by In Situ Hybridization and Immunohistochemistry MethodsJosé María Martín-Durán
15. Whole-Mount BrdU Staining with Fluorescence In Situ Hybridization in PlanariansLi-Chun Cheng and Alejandro Sánchez Alvarado
16. Detection of Apoptotic Cells in Planarians by Whole-Mount TUNELBrad Stubenhaus and Jason Pellettieri
17. Systemic RNA Interference in Planarians by Feeding of dsRNA Containing BacteriaCarolyn E. Adler and Alejandro Sánchez Alvarado
18. RNA Interference in Planarians: Feeding and Injection of Synthetic dsRNANorito Shibata and Kiyokazu Agata
19. A Subtractive FACS Method for Isolation of Planarian Stem Cells and Neural CellsTetsutaro Hayashi and Kiyokazu Agata
20. Clonal Analysis of Planarian Stem Cells by Subtotal Irradiation and Single-Cell TransplantationIrving E. Wang, Daniel E. Wagner, and Peter W. Reddien
21. Tissue Transplantations in PlanariansJosé I. Rojo-Laguna and Emili Saló
22. Live Imaging PlanariaWei Shen, Yun Shen, Yun Wah Lam, and Danny Chan
23. Processing Schmidtea mediterranea for Transmission Electron Microscopy: Classical and Microwave TechniquesJohn L. Brubacher, Ana P. Vieira, and Juliette Azimzadeh
24. Tissue Extracts for Quantitative Mass Spectrometry of Planarian Proteins Using SILACAlexander Böser, Hannes C.A. Drexler, and Kerstin Bartscherer
25. Quantification of Neurotransmitters from Intact and Regenerating Planarians Using UHPLC-MS/SRM MethodKannan Rangiah and Dasaradhi Palakodeti

Über Autor(innen)

Jochen Rink, PhD
Group Leader
Max Planck Institute of Molecular Cell Biology and Genetics
Pfotenhauerstrasse 108, 01307 Dresden, Germany
Tel: [phone]
Email: [e-mail]