RNA-Binding Proteins Part A

Erscheinungsjahr 2027
ISBN: 978-0-443-47334-0
Verlag: Elsevier Science & Technology

Buch, Englisch, Format (B × H): 152 mm x 229 mm, Gewicht: 450 g

Overall, this volume serves as a comprehensive methodological resource that integrates RNA-binding proteins, RNA metabolism, phase separation, and neurodegenerative disease mechanisms. As Volume 743 of the Methods in Enzymology series, RNA-Binding Proteins highlights recent advances in the field through chapters covering topics such as RNA-Binding Proteins: Structure, Function, and RNA Interactions; RNA Granules, Phase Separation, and Biomolecular Condensates; RNA Regulation in Cellular Physiology; RNA-Binding Proteins in Neurobiology and Neurodegeneration; RNA Processing, Editing, Splicing, and Decay; Non-Coding RNAs, Small RNAs, and RNA Therapeutics; and Experimental and Computational Methods for RNA–Protein Interaction Analysis. Collectively, these contributions provide valuable methodological and conceptual insights, making the volume highly relevant to researchers in molecular biology, neuroscience, RNA biology, and translational medicine.

Autoren/Hrsg.

Weitere Mitwirkende

Christianson, David

Allen, Karen N.

Chinnathambi, Subashchandrabose

Fachgebiete

• Naturwissenschaften Biowissenschaften Biochemie (nichtmedizinisch)
• Naturwissenschaften Biowissenschaften Molekularbiologie
• Naturwissenschaften Physik Angewandte Physik Biophysik

Inhaltsverzeichnis

1. Analysis of the dynamics of RNA-binding proteins in neuronal stress granules by quantitative live cell imaging
Roland Brandt
2. Quantitative Analysis of A-to-I Editing and Hyper-editing in Human and Mouse Data
Quentin Vicens, Mary Ifeoluwa Adesina, Kent Riemondy, Beat Vögeli and Parker Nichols
3. Phosphorylation Regulates CIRBP Arginine Methylation, Transportin-1 Binding and Liquid-Liquid Phase Separation
Tobias Madl
4. RNA editing of AZIN1 coding sites is catalyzed by ADAR1 p150 after splicing
Yukio Kawahara
5. RNA-binding proteins in synaptic plasticity
Sourav Banerjee
6. CPEB4-repressor complex-mediated mRNA degradation
Georg Stoecklin
7. Factors governing the binding affinity of protein-RNA complexes and their mutants analysis and prediction
M. Michael Gromiha
8. CX3CR1-mediated extracellular Tau internalization and phagosomal proteins in iPSC-derived microglia
Subashchandrabose Chinnathambi, Vaishnavi Ananthanaraya and Tanu Suma Narayanappa
9. RbBP5 ß-propeller domain reveals a surface with potential nucleic acid binding sites
Anshumali Mittal
10. Experimental Strategies for Studying RNA-Binding Proteins and Their Complexes in Alternative mRNA Splicing
Ashish Misra
11. Differential conformational dynamics in two type-A RNA-binding domains drive the double-stranded RNA recognition and binding
Jeetender Chugh
12. Modelling pathophysiological functions of TDP-43 protein in C. elegans
Ghulam Jeelani Pir
13. Biophysical Characterization of alpha-synuclein phase separation in vitro
Samir K. Maji
14. Design, generation and analysis of RNA substrates for biochemical deconstruction of histone mRNA decay
Sutapa Chakrabarti and Alexandrina Machado de Amorim
15. Liquid-to-solid phase transition of oskar ribonucleoprotein granules is essential for their function in Drosophila embryonic development
Mainak Bose
16. RNA Aptamers Rescue Mitochondrial Dysfunction in Huntington’s Disease
Ipsita Roy and Prajjwal Kushwaha
17. An in vitro quantitative PCR based technique for accurate measurement of RNA cap status
Chandrama Mukherjee and Avik Mukherjee
18. Liquid-liquid phase separation (LLPS) of proteins and RNA molecules
Kanchan Garai
19. Emerging role of circular RNAs in pancreatic ß cells
Amaresh Chandra Panda
20. MicroRNAs from extracellular vesicles as a signature for Parkinson's disease
Katrin Marcus-Alic
21. The Molecular Function of DEAD- and DExH-Box ATPases and Their Complex Relationship with Membraneless Organelles
Maria Hondele

Über Autor(innen)

Chinnathambi, Subashchandrabose
Subashchandrabose Chinnathambi is Additional Professor at the Department of Neurochemistry, National Institute of Mental Health and Neurosciences Hospital Bangalore, Karnataka, India. His scientific interests include physiopathology of neurodegenerative disorders, Tau Cytoskeleton, Tau-GPCR, purinergic and chemokine receptors, Tau stem cells, animal models, neuropharmacology, therapeutic approaches targeting Tau oligomers, and biomarkers for neurodegeneration. He serves on the editorial board for various international cell biology journals and is also a regular invited speaker at a number of meetings and workshops. Dr. Chinnathambi has more than 16 years of research experience in the area of neuroscience and Tau-related research in Alzheimer’s Disease.

Allen, Karen N.
Dr. Karen N. Allen works at the Department of Chemistry of the Boston University, the Metcalf Center for Science and Engineering

Christianson, David
After completing studies for the A.B., A.M., and Ph.D. degrees in chemistry at Harvard University, David W. Christianson joined the faculty of the University of Pennsylvania, where he is currently the Roy and Diana Vagelos Professor in Chemistry and Chemical Biology. At Penn, Christianson’s research focuses on the structural and chemical biology of the zinc-dependent histone deacetylases as well as enzymes of terpene biosynthesis. His research accomplishments have been recognized by several awards, including the Pfizer Award in Enzyme Chemistry and the Repligen Award in Chemistry of Biological Processes from the American Chemical Society, a Guggenheim Fellowship, and the Elizabeth S. and Richard M. Cashin Fellowship from the Radcliffe Institute for Advanced Study at Harvard University. Christianson is also a dedicated classroom teacher, and his accomplishments in this regard have been recognized by the Lindback Award for Distinguished Teaching at Penn and a Rhodes Trust Inspirational Educator Award from Oxford University. Christianson has also held visiting professorships in the Department of Biochemistry at Cambridge University and the Department of Chemistry and Chemical Biology at Harvard University. Christianson has served with Prof. Anna Pyle as Co-Editor-in-Chief of Methods in Enzymology since 2015.