RNA-Binding Proteins Part D

Erscheinungsjahr 2027
ISBN: 978-0-443-47464-4
Verlag: Elsevier Science & Technology

Buch, Englisch, Format (B × H): 152 mm x 229 mm, Gewicht: 450 g

A central theme of this volume is the dynamic relationship between RNA structure, RNA-binding proteins, and cellular organization. The chapters integrate experimental, computational, biochemical, biophysical, imaging, and systems-level approaches to investigate how RNA–protein interactions regulate RNA processing, translation, localization, stability, phase separation, and cellular stress responses. As Volume 746 of the Methods in Enzymology series, the volume emphasizes the integration of methodological innovation with biological applications, providing researchers with practical approaches to dissect how RNA–protein interactions govern RNA fate and function, biomolecular condensate formation, and stress granule assembly.

Overall, this collection highlights the transition of RNA biology from a primarily sequence-centered discipline toward a more dynamic view in which RNA structure, molecular interactions, phase separation, cellular organization, and signaling are intimately connected. It will therefore be a valuable resource for researchers and students seeking both practical methodologies and mechanistic insights into the rapidly expanding field of RNA biology.

Autoren/Hrsg.

Weitere Mitwirkende

Christianson, David

Allen, Karen N.

Chinnathambi, Subashchandrabose

Fachgebiete

• Naturwissenschaften Biowissenschaften Biochemie (nichtmedizinisch)
• Naturwissenschaften Physik Angewandte Physik Biophysik
• Naturwissenschaften Biowissenschaften Molekularbiologie

Inhaltsverzeichnis

1. RNA Recognition and Stress Granule Assembly Mediated by TIA Proteins
Irene Diaz-Moreno and Ana Belén Uceda-Mayo
2. The effect of G-quadruplexes on TDP43 condensation, distribution, and toxicity
Scott Horowitz
3. Focal adhesion-derived liquid-liquid phase separations regulate mRNA translation
Abhishek Kumar
4. Experimental Approaches to detect SARS-CoV-2-UTR-RNA-Protein Interactome in mammalian cells
Milan Surjit, Rohit Verma and Sreemoyee Ghosh
5. Mechanistic investigation of CX3CR1-mediated Tau degradation via mTOR in microglial cells
Subashchandrabose Chinnathambi and Vaishnavi Ananthanarayana
6. Conformations and Dynamics of dsRNA Binding Proteins Regulate Substrate Selection
Mandar V. Deshmukh
7. RNA-binding proteins and its processing
Hem Chandra Jha, Vaishali Saini, Srija Mukherjee, Chaitali Vora and Himanshi Jindal
8. RNA-binding proteins and its activity
Bikash Choudhary
9. RNA G-quadruplex structures: Detection and binding to RBPs
Purusharth Rajyaguru and Rocky Pachar
10. Experimental and Bioinformatic Workflow to Identify and Test Ribosome-Associated RNAs That Induce Prion Protein Phase Separation
Yraima Cordeiro, Jerson L. Silva, Ana C. Tahira, Maria Heloisa Freire, Marcelly Muxfeldt, Luciana P. Rangel and Sergio Verjovski-Almeida
11. Strategies for Dissecting the PIWI–piRNA–DNMT Axis: Epigenetic Silencing Mechanisms Underlying Tumorigenesis
Bibekanand Mallick, Sruti Biswal and Priyajit Biswal
12. microRNA, RNAi, suppressors of RNAi
Neeti Sanan-Mishra and Tilahun Rabuma
13. RNA-protein interaction and its network
Sucharita Dey and Rishabh Gaur
14. Structure and Dynamics of dsRNA Binding Proteins
Mandar V. Deshmukh
15. RNA-protein interaction and its network
Arul Murugan Natarajan Sr.
16. RNA-binding Proteins and its processing by biomolecular simulation
Ramasubbu Sankararamakrishnan
17. Protein-Nucleic Acid Interactions by NMR
Sai Chaitanya Chiliveri
18. Biophysical methods for the characterization of lncRNA Ribosnitches and their interaction with binding proteins
Santosh Kumar
19. Biophysical and molecular methods to identify and understand the relationship between RNA-binding proteins with different types of RNA modifications
Santosh Kumar, Smruti Rekha Biswal, Kirthik Roshan and Srinivasan Muthuswamy
20. RNA-Binding Proteins and Non-Coding RNAs in Epigenetic Regulation: Implications for Hematopoiesis and Leukemia
Ravi Kumar Gutti
21. A comprehensive workflow for miRNA profiling in human brain and blood samples
MM Srinivas Bharath
22. Evolutionary analysis of conserved and non-conserved sub-regions in a protein with Vertical View
Klaus Förstemann

Über Autor(innen)

Chinnathambi, Subashchandrabose
Subashchandrabose Chinnathambi is Additional Professor at the Department of Neurochemistry, National Institute of Mental Health and Neurosciences Hospital Bangalore, Karnataka, India. His scientific interests include physiopathology of neurodegenerative disorders, Tau Cytoskeleton, Tau-GPCR, purinergic and chemokine receptors, Tau stem cells, animal models, neuropharmacology, therapeutic approaches targeting Tau oligomers, and biomarkers for neurodegeneration. He serves on the editorial board for various international cell biology journals and is also a regular invited speaker at a number of meetings and workshops. Dr. Chinnathambi has more than 16 years of research experience in the area of neuroscience and Tau-related research in Alzheimer’s Disease.

Allen, Karen N.
Dr. Karen N. Allen works at the Department of Chemistry of the Boston University, the Metcalf Center for Science and Engineering

Christianson, David
After completing studies for the A.B., A.M., and Ph.D. degrees in chemistry at Harvard University, David W. Christianson joined the faculty of the University of Pennsylvania, where he is currently the Roy and Diana Vagelos Professor in Chemistry and Chemical Biology. At Penn, Christianson’s research focuses on the structural and chemical biology of the zinc-dependent histone deacetylases as well as enzymes of terpene biosynthesis. His research accomplishments have been recognized by several awards, including the Pfizer Award in Enzyme Chemistry and the Repligen Award in Chemistry of Biological Processes from the American Chemical Society, a Guggenheim Fellowship, and the Elizabeth S. and Richard M. Cashin Fellowship from the Radcliffe Institute for Advanced Study at Harvard University. Christianson is also a dedicated classroom teacher, and his accomplishments in this regard have been recognized by the Lindback Award for Distinguished Teaching at Penn and a Rhodes Trust Inspirational Educator Award from Oxford University. Christianson has also held visiting professorships in the Department of Biochemistry at Cambridge University and the Department of Chemistry and Chemical Biology at Harvard University. Christianson has served with Prof. Anna Pyle as Co-Editor-in-Chief of Methods in Enzymology since 2015.