Macromolecular Crystallography

conventional and high-throughput methods
Erscheinungsjahr 2007
ISBN: 978-0-19-852097-9
Verlag: Oxford University Press

Buch, Englisch, 292 Seiten, Format (B × H): 194 mm x 253 mm, Gewicht: 808 g

Macromolecular Crystallography is the study of macromolecules (proteins and nucleic acids) using X-ray crystallographic techniques in order to determine their molecular structure. The knowledge of accurate molecular structures is a pre-requisite for rational drug design, and for structure-based function studies to aid the development of effective therapeutic agents and drugs.

The successful determination of the complete genome (genetic sequence) of several species (including humans) has recently directed scientific attention towards identifying the structure and function of the complete complement of proteins that make up that species; a new and rapidly growing field of study called 'structural genomics'. There are now several important and well-funded global initiatives in operation to identify all of the proteins of key model species. One of the main requirements for these initiatives is a high-throughput crystallization facility to speed-up the protein identification process. The extent to which these technologies have advanced, calls for an updated review of current crystallographic theory and practice.

This practical reference book features the latest conventional and high-throughput methods, and includes contributions from a team of internationally recognized leaders and experts. It will be of relevance and use to graduate students, research scientists and professionals currently working in the field of conventional and high-throughput macromolecular crystallography.

Zielgruppe

Research scientists, graduate students and professionals currently working with, or interested in working in the field of conventional and high-throughput macromolecular crystallography.

Autoren/Hrsg.

Sanderson, Mark R.

Skelly, Jane V.

Fachgebiete

• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Vorklinische Medizin: Grundlagenfächer Humangenetik
• Naturwissenschaften Biowissenschaften Biochemie (nichtmedizinisch)
• Naturwissenschaften Physik Thermodynamik Festkörperphysik, Kondensierte Materie
• Naturwissenschaften Biowissenschaften Molekularbiologie
• Naturwissenschaften Chemie Physikalische Chemie Chemische Kristallographie

Inhaltsverzeichnis

- 1: Skelly, Sohi and Batuwangala: Classical Cloning, Expression and Purification

- 2: Owens, Nettleship, Berrow, Stuart and Stammers: High-Throughput Cloning, Expression and Purification

- 3: Chayen: Automation of Non-Conventional Crystallization Techniques for Screening and Optimisation

- 4: Abdel-Meguid, Jeruzalmi and Sanderson: First Analysis of Macromolecular Crystals

- 5: Sanderson: In-House Macromolecular Data Collection

- 6: Abdel-Meguid: Solving the Phase Problem Using Isomorphous Replacement

- 7: Delarue: Molecular Replacement Techniques for High-Throughput Structure Determination

- 8: Murthy: MAD Phasing

- 9: Weeks and Furey: Application of Direct Methods to Macromolecular Structure Solution

- 10: Abrahams: Phase Refinement through Density Modification

- 11: Morris, Perrakis and Lamzin: Getting a Macromolecular Model: Model Building, Refinement and Validation

- 12: Wasserman, Smith, D'Amico, Koss, Morisco, and Burley: High-Throughput Crystallographic Data Collection at Synchrotrons

- 13: Carson: Electron Density Fitting and Structure Validation

- 14: Masquida, Francois, Werner and Westhof: RNA Crystallogenesis

- 15: Sohi and Laponogov: Crystallography in the Study of Protein-DNA Interaction

- 16: Fry, Abrescia and Stuart: Virus Crystallography

- 17: Abdel-Meguid: Macromolecular Crystallography in Drug Design

Über Autor(innen)

Mark Sanderson is an experienced protein and nucleic-acid crystallographer and co-editor of Methods in Molecular Biology, who has solved several key ab initio structures, notably nucleic-acid proteins.

Jane Skelly has widespread experience in the expression and crystallisation of macromolecules.
Both editors lecture undergraduates in structural biology.