Buch, Englisch, Band 1726, 200 Seiten, Previously published in hardcover, Format (B × H): 178 mm x 254 mm, Gewicht: 409 g
Reihe: Methods in Molecular Biology
Buch, Englisch, Band 1726, 200 Seiten, Previously published in hardcover, Format (B × H): 178 mm x 254 mm, Gewicht: 409 g
Reihe: Methods in Molecular Biology
ISBN: 978-1-4939-8522-7
Verlag: Springer
This volume covers the mechanisms of pRb inactivation detailing repressive mechanisms commonly associated to cancer, and representative of the experimentally relevant tests used in the establishment of cancer diagnosis and prognosis. Chapters contain protocols and in-depth discussions for commonly used experimental approaches to assess the status and function of components of the pRb pathway, including pRb itself, in cell lines and biological samples.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, The Retinoblastoma Protein aims to serve as a guide to assist molecular cancer biologists in their search for understanding of the molecular functions of this preeminent tumor suppressor.
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Weitere Infos & Material
1.Characterization of RB1 Deletions in Interphase and Metaphase by Molecular Cytogenetics Exemplified in Chronic Lymphatic Leukemia
Thomas Liehr
2.Detection of RB1 Gene Copy Number Variations using a Multiplex Ligation-Dependent Probe Amplification Method
Nejat Dalay
3.A Fluorescent Quantitative Multiplex PCR Method to Detect Copy Number Changes in the RB1 Gene
Chitra Kannabiran
4. Using Methylation-specific PCR to study RB1 Promoter Hypermethylation
Thaís M. McCormick and Maria da Glória da C. Carvalho
5. Detection of Aberrant DNA Methylation Patterns in the RB1 GeneSumadi Lukman Anwar and Ulrich Lehmann
6. Detection of Retinoblastoma Protein Phosphorylation by Immunoblot Analysis
Pedro G. Santiago-Cardona, Jaileene Pérez-Morales, and Jonathan González-Flores7. Immunohistochemical Detection of the Retinoblastoma Protein
Charles A. Ishak, Matthew J. Cecchini, Christopher J. Howlett, and Frederick A. Dick
8. Immunohistochemical Detection of Retinoblastoma Protein Phosphorylation in Human Tumor Samples
Jaileene Pérez-Morales, Angel Núñez-Marrero, and Pedro G. Santiago-Cardona
9. Detection of CCND1 Locus Amplification by Fluorescence in situ HybridizationMargit Balázs, Viktória Koroknai, István Szász, and Szilvia Ecsedi
10. Detection of CCND1 Gene Copy Number Variations using Multiplex Ligation-dependent Probe Amplification and Fluorescence in situ Hybridization Methods
Akishi Ooi and Takeru Oyama
11. Detection of p16 Promoter Hypermethylation by Methylation-specific PCR
Javed Hussain Choudhury, Raima Das, Shaheen Laskar, Sharbadeb Kundu, Manish Kumar,
Partha Pratim Das, Yashmin Choudhury, Rosy Mondal, and Sankar Kumar Ghosh
12. Immunohistochemical Detection of p16 in Clinical Samples
Georgia Karpathiou and Michel Peoc'h
13. Detection of E2F-DNA Complexes Using Chromatin Immunoprecipitation Assays
Miyoung Lee, Lorraine J. Gudas, and Harold I. Saavedra
14. Detection of E2F-induced Transcriptional Activity Using a Dual Luciferase Reporter Assay
Ainhoa Iglesias-Ara, Nerea Osinalde, and Ana M. Zubiaga
15. Detection of HPV E6/E7 mRNA in Clinical Samples using RNA in situ HybridizationManishkumar Pandey, Priyanka G. Bhosale, and Manoj B. Mahimkar
16. CRISPR/Cas9 Mediated Knockout of Rb1 in Xenopus tropicalis
Thomas Naert and Kris Vleminckx
