Buch, Englisch, 290 Seiten, Format (B × H): 183 mm x 260 mm, Gewicht: 708 g
Reihe: Methods in Molecular Biology
Methods and Protocols
Buch, Englisch, 290 Seiten, Format (B × H): 183 mm x 260 mm, Gewicht: 708 g
Reihe: Methods in Molecular Biology
ISBN: 978-1-0716-5554-2
Verlag: Springer-Verlag New York Inc.
This fully updated volume collects relevant and recent experimental approaches using atomic force microscopy (AFM) in biology and biomedical sciences. Beginning by addressing AFM imaging of biological samples and common practical considerations, the book continues by covering single molecule, molecular recognition approaches, protocols focused on membranes and bacteria, viruses and protein cages, and AFM nanomechanics and biomedical applications. Written for the highly successful series, chapters include introductions to their respective topics, lists of necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding common pitfalls.
Authoritative and up-to-date, serves as a valuable reference for using AFM on biological and biomedical samples, AFM sample preparation, data acquisition and processing, as well as tips and tricks for optimizing AFM measurements.
Zielgruppe
Professional/practitioner
Autoren/Hrsg.
Fachgebiete
Weitere Infos & Material
Artifacts and Practical Issues in Atomic Force Microscopy.- Imaging Artificial Membranes Using High-Speed Atomic Force Microscopy.- Analysis of DNA-Protein Complexes by Atomic Force Microscopy Imaging: The Case of TRF2-Telomeric DNA Wrapping.- Functionalization of AFM Tips and Supports for Molecular Recognition Force Spectroscopy and Recognition Imaging.- Single-Molecule Force Spectroscopy: Experiments, Analysis, and Simulations.- AFM to Study Pore-Forming Proteins.- Investigation of Bacterial Curli Production and Adhesion Using AFM.- Effects of Antimicrobial Peptides on Bacterial Cells.- Protocol for AFM Nanoindentation of Viruses and Protein Cages.- Physical Virology with Atomic Force and Fluorescence Microscopies: Seeing and Touching Viruses and Protein Cages.- Quantification of the Elastic Properties of Soft and Sticky Materials Using AFM.- Measuring the Elastic Properties of Living Cells.- Molecular Recognition Force Spectroscopy for Probing Cell Targeted Nanoparticles In Vitro.- Enhancing Biomedical Implants: Atomic Force Microscopy Insights into Modified Titanium Surfaces.- Biomechanical Characterization of Human Pluripotent Stem Cell-Derived Cardiomyocytes by Use of Atomic Force Microscopy.
