Protein Interactions

Biophysical Approaches for the Study of Complex Reversible Systems
1. Auflage Softcover of orig. Auflage 2007
ISBN: 978-1-4419-4208-1
Verlag: Springer US

Buch, Englisch, 532 Seiten, Previously published in hardcover, Format (B × H): 155 mm x 235 mm, Gewicht: 814 g

Reihe: Protein Reviews

This volume successfully and clearly examines how biophysical approaches can be used to study complex systems of reversibly interacting proteins. It deals with the methodology behind the research and shows how to synergistically incorporate several methodologies for use. Each chapter treats and introduces the reader to different biological systems, includes a brief summary of the physical principles, and mentions practical requirements.

Zielgruppe

Research

Autoren/Hrsg.

Schuck, Peter

Fachgebiete

• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Vorklinische Medizin: Grundlagenfächer Physik, Chemie, Biologie für Mediziner
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Vorklinische Medizin: Grundlagenfächer Biochemie (med.)
• Naturwissenschaften Physik Angewandte Physik Biophysik
• Naturwissenschaften Biowissenschaften Angewandte Biologie Biophysik
• Naturwissenschaften Biowissenschaften Zellbiologie
• Naturwissenschaften Biowissenschaften Proteinforschung
• Naturwissenschaften Physik Angewandte Physik Medizinische Physik

Inhaltsverzeichnis

The Characterization of Biomolecular Interactions Using Fluorescence Fluctuation Techniques.- Characterization of Protein–Protein Interactions Using Atomic Force Microscopy.- Combined Solid-Phase Detection Techniques for Dissecting Multiprotein Interactions on Membranes.- Surface Plasmon Resonance Biosensing in the Study of Ternary Systems of Interacting Proteins.- Mass Spectrometry for Studying Protein Modifications and for Discovery of Protein Interactions.- H/2H Exchange Mass Spectrometry of Protein Complexes.- Elucidation of Protein–Protein and Protein–Ligand Interactions by NMR Spectroscopy.- Application of Isothermal Titration Calorimetry in Exploring the Extended Interface.- Solvent Mediated Protein–Protein Interactions.- Sedimentation Equilibrium Analytical Ultracentrifugation for Multicomponent Protein Interactions.- Structure Analysis of Macromolecular Complexes by Solution Small-Angle Scattering.- Fluorescence Detection of Proximity.- Steady-State and Time-Resolved Emission Anisotropy.- Analysis of Protein-DNA Equilibria by Native Gel Electrophoresis.- Electrospray Ionization Mass Spectrometry and the Study of Protein Complexes.- Sedimentation Velocity in the Study of Reversible Multiprotein Complexes.

Über Autor(innen)

Dr. Peter Schuck is the acting chief of the Protein Biophysics department in the National Institute of Health’s Division of Bioengineering and Physical Science. His current research work is on protein structure and function, with studies in multi-protein complexes, membrane proteins in detergent solution, self-assembling and filamenting proteins, structural and nonstructural viral proteins, homogeneous and heterogeneous interactions of immunological cell surface receptors; and biophysical methods and instrumentation through analytical ultracentrifugation, optical biosensors, static and dynamic light scattering, calorimetry, and circular dichroism.