Buch, Englisch, 317 Seiten, Format (B × H): 183 mm x 260 mm, Gewicht: 821 g
Reihe: Methods in Molecular Biology
Methods and Protocols
Buch, Englisch, 317 Seiten, Format (B × H): 183 mm x 260 mm, Gewicht: 821 g
Reihe: Methods in Molecular Biology
ISBN: 978-1-0716-4745-5
Verlag: Springer US
This volume presents a comprehensive collection of protocols and techniques commonly used in research on plant-biotic interactions. Chapters guide readers through general plant–pathogen interactions, RNA interference (RNAi) in , spot inoculation of begomoviruses, natural transmission, RNA viruses, enzyme-linked immunosorbent, reverse transcription polymerase chain reaction (RT-PCR), loop-mediated isothermal amplification (RT-LAMP), northern blot hybridization, and next-generation sequencing (NGS) for in-depth analysis of viral populations and mutation patterns. Written in the highly successful series format, the chapters include brief introductions tothe material, lists of necessary materials and reagents, step-by-step, readilyreproducible laboratory protocols, and a Notes section which highlightstips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, aims to be comprehensive guide forresearchers in the field.
Zielgruppe
Professional/practitioner
Autoren/Hrsg.
Weitere Infos & Material
Plant Biotic Stress: Tools and Techniques for Crop Protection.- Investigating the Involvement of Target Protein in Plant-Pathogen Interactions through Ectopic Expression in Arabidopsis – A detailed methodology.- RNA interference strategy for the development of okra (Abelmoschus esculentus L. Moench) lines resistant to Yellow vein mosaic virus.- Spot inoculation of begomovirus by single whitefly (Bemisia tabaci) transmission in mungbean using microcage.- One-Step RT-LAMP: A Method for Closed-Tube Colorimetric Detection of RNA Viruses in Plants.- Detection of Sesame Phyllody Disease and Its Transmitting Vectors.- A method for isolation and transfection of Arabidopsis protoplast for sucrose feeding assay.- Twitching motility dynamics in the wilt-causing phytopathogen Ralstonia solanacearum.- A blotting paper technique for the screening of chickpea genotypes against dry root rot disease.- CRISPR-mediated gene editing for inducing thermo-sensitive genic male sterility and sheath blight resistance in rice.- A Seedling Root Dip-based Technique to Screen Chickpea for Resistance to Fusarium Wilt Disease.- Oligo-dT Anchored cDNA SCoT: An Innovative Approach for Deciphering Fusarium wilt Resistance-Related Differential Gene Expression in Chickpea.- Extracellular acidification assay to evaluate the effectiveness of antifungal agents on the pathogenicity of Macrophomina phaseolina.- A guide to culturing, maintenance and leaf inoculation methods for rapid screening and quantification of Sclerotinia sclerotiorum infection in mustard.- Differential-display reverse transcription-PCR for unraveling the molecular mechanisms in foot rot (Phytophthora capsici sp. nov.) tolerance in black pepper (Piper nigrum L.).- Estimation of foliar volatiles emitted by mycorrhizal colonized blackgram (Vigna mungo L) infested with Spodoptera litura.- Development of an Agrobacterium tumefaciens-mediated in planta transformation strategy in pigeonpea for resistance to Helicoverpa armigera.- CRISPR/Cas9 mediated genome editing in the management of oriental fruit fly, Bactrocera dorsalis (Hendel) (Tephritidae: Diptera).- A novel approach using SSR markers for identifying and breeding Bruchid pest resistant black gram (Vigna mungo L. Hepper) genotypes.- RNAi-based approach as a promising tool for management of leafhopper (Amrasca biguttula biguttula) in cotton.- Germplasm evaluation in chickpea for resistance to plant parasitic nematodes.- Novel method for rapid screening of chickpea for combined dry root rot disease and osmotic stress.
