Molecular and Cell Biology Methods for Fungi

Softcover Nachdruck of the original 1. Auflage 2010
ISBN: 978-1-4939-6146-7
Verlag: Humana Press

Buch, Englisch, Band 638, 321 Seiten, Paperback, Format (B × H): 178 mm x 254 mm, Gewicht: 634 g

Reihe: Methods in Molecular Biology

The kingdom Fungi constitutes an independent group equal in rank to that of plants and animals. It is a diverse clade of heterotrophic eukaryotic organisms that shares some ch- acteristics with animals and includes mushrooms, molds, yeasts as well as many other types of less well known organisms. Approximately 100,000 species have been described, which comprise less than 10% of the estimated number of fungal species in nature. Fungi can be found in every place wherever adequate moisture, temperature, and organic substrates are available; however, they also occupy extreme habitats, from hot volcanoes to arctic zones, arid deserts, and deep oceans. The importance of fungi as a group is tremendous; most species are saprobes and play prime roles in decomposition and the recycling of organic matter and nutrients, and many of them produce enzymes and metabolites with important applications in pharmacology, biotechnology, and other industries. Alongside the positive aspects, fungi also cause huge damage, primarily as plant pathogens. Fungi are highly amenable to molecular work, and a few fungal species serve as model systems to study basic processes with results that are applicable to many organisms, including humans.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Sharon, Amir

Fachgebiete

• Naturwissenschaften Biowissenschaften Botanik Mykologie, Pilze
• Naturwissenschaften Biowissenschaften Zellbiologie
• Naturwissenschaften Biowissenschaften Botanik Pflanzenreproduktion, Verbreitung, Genetik
• Naturwissenschaften Biowissenschaften Biochemie (nichtmedizinisch)
• Naturwissenschaften Biowissenschaften Biowissenschaften Genetik und Genomik (nichtmedizinisch)
• Naturwissenschaften Biowissenschaften Mikrobiologie

Inhaltsverzeichnis

Fungal Transformation and Gene Knockout.- Protoplast Transformation of Filamentous Fungi.- Electroporation and Agrobacterium-Mediated Spore Transformation.- High-Throughput Construction of Gene Deletion Cassettes for Generation of Neurospora crassa Knockout Strains.- Development of Impala-Based Transposon Systems for Gene Tagging in Filamentous Fungi.- DelsGate: A Robust and Rapid Method for Gene Deletion.- Gene Silencing for Functional Analysis: Assessing RNAi as a Tool for Manipulation of Gene Expression.- Detection and Quantification of Fungi.- Analysis of Fungal Gene Expression by Real Time Quantitative PCR.- Identification of Differentially Expressed Fungal Genes In Planta by Suppression Subtraction Hybridization.- Quantification of Fungal Infection of Leaves with Digital Images and Scion Image Software.- Expression Profiling of Fungal Genes During Arbuscular Mycorrhiza Symbiosis Establishment Using Direct Fluorescent In Situ RT-PCR.- Application of Laser Microdissection to Study Plant–Fungal Pathogen Interactions.- Multiplex Gene Expression Analysis by TRAC in Fungal Cultures.- Amplification of Fungal Genomes Using Multiple Displacement Amplification.- Microscopy and Protein Analysis.- Biochemical Methods Used to Study the Gene Expression and Protein Complexes in the Filamentous Fungus Neurospora crassa.- Measuring Protein Kinase and Sugar Kinase Activity in Plant Pathogenic Fusarium Species.- A Detailed Protocol for Chromatin Immunoprecipitation in the Yeast Saccharomyces cerevisiae.- A Method to Visualize the Actin and Microtubule Cytoskeleton by Indirect Immunofluorescence.- Fluorescence In Situ Hybridization for Molecular Cytogenetic Analysis in Filamentous Fungi.- Live-Cell Imaging of Microtubule Dynamics in Hyphae of Neurospora crassa.- Methods to DetectApoptotic-Like Cell Death in Filamentous Fungi.- Evaluation of Antifungal Susceptibility Using Flow Cytometry.- Preparation of Fungi for Ultrastructural Investigations and Immunogoldlabelling.- Split-EGFP Screens for the Detection and Localisation of Protein–Protein Interactions in Living Yeast Cells.