Phage Display in Biotechnology and Drug Discovery

2. Auflage 2014
ISBN: 978-1-4398-3649-1
Verlag: CRC Press

Buch, Englisch, 584 Seiten, Format (B × H): 172 mm x 237 mm, Gewicht: 925 g

Reihe: Drug Discovery Series

Phage Display in Biotechnology and Drug Discovery, Second Edition provides a comprehensive view of the impact and promise of phage display in drug discovery and biotechnology. Building on the success of its previous edition, the book discusses current theories, principles, and methods in the field and demonstrates applications for peptide phage display, protein phage display, and the development of novel antibodies.

The book provides readers with an overview of the amazing breadth of the impact that phage display technology has had on the study of proteins in general as well as the development of proteins. It will be a valuable resource for those interested in using phage display and recombinant antibodies in basic research and drug discovery.

Zielgruppe

Graduate, postdoctoral and senior researchers in the fields of therapeutic biotechnology and protein engineering and researchers interested in recombinant antibodies.

Autoren/Hrsg.

Sidhu, Sachdev S

Geyer, Clarence Ronald

Fachgebiete

• Interdisziplinäres Wissenschaften Wissenschaften: Forschung und Information Forschungsmethodik, Wissenschaftliche Ausstattung
• Naturwissenschaften Chemie Chemie Allgemein
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Pharmazie

Inhaltsverzeichnis

Filamentous Bacteriophage Structure and Biology. Vectors and Modes of Display. Methods for the Construction of Phage-Displayed Libraries. Selection and Screening Strategies. Leveraging Synthetic Phage-Antibody Libraries for Panning on the Mammalian Cell Surface. Phage Libraries for Developing Antibody-Targeted Diagnostics and Vaccines. Exploring Protein-Protein Interactions Using Peptide Libraries Displayed on Phage. Substrate Phage Display. Mapping Intracellular Protein Networks. High-Throughput and High-Content Screening Using Peptides. Engineering Protein Folding and Stability. Identification of Natural Protein-Protein Interactions with cDNA Libraries. Mapping Protein Functional Epitopes. Selections for Enzymatic Catalysts. Antibody Humanization and Affinity Maturation Using Phage Display. Antibody Libraries from Immunized Repertoires. Naive Antibody Libraries from Natural Repertoires. Synthetic Antibody Libraries. Engineering Antibody Fragments for Intracellular Applications.

Über Autor(innen)

Edited by

Sachdev S. Sidhu, PhD, professor, Department of Molecular Genetics, University of Toronto, Ontario, Canada

C. Ronald Geyer, PhD, professor, Department of Pathology, University of Saskatchewan, Canada