Genome Engineering via CRISPR-Cas9 System

Erscheinungsjahr 2020
ISBN: 978-0-12-818140-9
Verlag: William Andrew Publishing

Buch, Englisch, 384 Seiten, Format (B × H): 216 mm x 276 mm, Gewicht: 930 g

Genome Engineering via CRISPR-Cas9 Systems presents a compilation of chapters from eminent scientists from across the globe who have established expertise in working with CRISPR-Cas9 systems. Currently, targeted genome engineering is a key technology for basic science, biomedical and industrial applications due to the relative simplicity to which they can be designed, used and applied. However, it is not easy to find relevant information gathered in a single source. The book contains a wide range of applications of CRISPR in research of bacteria, virus, algae, plant and mammalian and also discusses the modeling of drosophila, zebra fish and protozoan, among others.

Other topics covered include diagnosis, sensor and therapeutic applications, as well as ethical and regulatory issues. This book is a valuable source not only for beginners in genome engineering, but also researchers, clinicians, stakeholders, policy makers, and practitioners interested in the potential of CRISPR-Cas9 in several fields.

Zielgruppe

<p>Researchers in genome engineering, synthetic biology, metabolic engineering</p>

Autoren/Hrsg.

Singh, Vijai

Dhar, Pawan K.

Fachgebiete

• Naturwissenschaften Biowissenschaften Bakteriologie
• Technische Wissenschaften Verfahrenstechnik | Chemieingenieurwesen | Biotechnologie Biotechnologie
• Naturwissenschaften Biowissenschaften Biowissenschaften Genetik und Genomik (nichtmedizinisch)
• Naturwissenschaften Biowissenschaften Biowissenschaften DNA und Transgene Organismen

Inhaltsverzeichnis

1. An introduction to genome editing CRISPR-Cas systems2. Evolution and mechanism of CRISPR-Cas9 systems3. Exploring the potential of CRISPR-Cas9 for removal of human viruses4. Programmable removal of bacterial pathogens using CRISPR-Cas9 system5. Targeted genome editing using CRISPR-Cas9 system in fungi6. CRISPR-Cas9 system for fungi genome engineering towards industrial applications7. Development and challenges of using CRISPR-Cas9 system in mammalians8. CRISPR technology for cell line development and engineering9. Exploring the potential of genome editing CRISPR-Cas9 system in cancer 10. CRISPR-Cas9 for therapy: the challenges and ways to overcome them11. Engineered Cas9 nucleases12. The current progress of CRISPR/Cas9 development in plants13. Fruit crops improvement using CRISPR-Cas9 system14. CRISPR-Cas9 engineered viral immunity in plants15. Genome engineering in medicinally important plants using CRISPR/Cas9 tool16. Genome editing of algal species by CRISPR Cas9 for biofuels17. Development and use of CRISPR in industrial applications18. CRISPR interference based gene silencing in bacteria: Advantages and limitations19. Genome engineering in insects: focus on the CRISPR/Cas9 system20. Recent Progress of CRISPR- Cas9 in Zebra Fish21. CRISPR: a revolutionary tool for genome engineering in the protozoan parasites22. Biosafety, biosecurity, patents, and regulatory issues

Über Autor(innen)

Singh, Vijai
Prof. Vijai Singh is currently serving as Head of the Department of Biosciences, School of Science and Dean (Research & Innovation) at Indrashil University, Rajpur, Mehsana, India. Prior to this, he held the position of Associate Professor in the same department at Indrashil University. He has also served as Assistant Professor in the Department of Biological Sciences and Biotechnology at the Institute of Advanced Research, Gandhinagar, and in the Department of Biotechnology at the Invertis University, Bareilly, India. Earlier of his career, Prof. Singh worked as a Postdoctoral Fellow in the Synthetic Biology Group at the Institute of Systems and Synthetic Biology, Paris, France and at the School of Energy & Chemical Engineering at the Ulsan National Institute of Science and Technology, Ulsan, South Korea. He earned his Ph.D. in Biotechnology in 2009 from the ICAR-National Bureau of Fish Genetic Resources, Uttar Pradesh Technical University, Lucknow, India. His doctoral research focused on the development of molecular and immunoassays for the diagnosis of Aeromonas hydrophila. Prof. Singh’s research interests lies in the design and construction of novel biosynthetic pathways for production of medically and industrially important biomolecules. His laboratory is also actively engaged in developing CRISPR-Cas systems for gene knock out, knock in, gene regulation and diagnostic. With over 15 years of research and teaching experience, his areas of expertise include synthetic biology, metabolic engineering, bioinformatics, microbiology, and industrial microbiology. He has an extensive academic record, with over 110 articles, 89 book chapters, 26 books and 3 patents to his credits. Prof. Singh has received number of prestigious awards, including the Bioclues Innovation, Research and Development Award (2023) from the BIOCLUES Society, the Vice Chancellor's Best Research Award (2023) from Indrashil University, and the Agathiyar Chemical Biology Award (2023) from the Society of Chemical and Synthetic Biology. At Indrashil University, he currently serves as chairman of the Board of Study, and is a member of the Academic Council of Indrashil University. He also holds the position of the Member Secretary of the Institutional Biosafety Committee (IBSC) at the university. Dr. Singh is actively involved in national and international scientific advisory roles.

Dhar, Pawan K.
Prof. Pawan K. Dhar is the Dean, School of Biotechnology, Jawaharlal Nehru University, New Delhi and heads an active synthetic biology group. Prior to this he held scientific positions at RIKEN Institute, Keio University and Kyoto University (Japan) Bioinformatics Institute, (Singapore) and Manipal University (India). Prof. Dhar received his Ph.D. in 1993 from Banaras Hindu University (Varanasi) for his work on Human Genetics. Prof Dhar’s lab holds specialization in making functional proteins from non-expressing DNA sequences leading to the development of a potentially groundbreaking drug discovery platform. Prof. Dhar serves in the external board of referees for European Science Foundation and Indian Government science funding agencies.