Smith Helicobacter Pylori

Methods and Protocols
1. Auflage 2021
ISBN: 978-1-07-161301-6
Verlag: Springer, Berlin

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Beschreibung

This book brings together a collection of methods for the successful detection, culture and investigation of H. pylori, and its associated diseases. Chapters guide the reader through overviews on H. pylori infection, protocols for analyzing human clinical samples, and protocols for characterizing H. pylori, its functional components and its interactions with host cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.



Authoritative and cutting-edge, Helicobacter pylori: Methods and Protocols aims to be a useful practical guide to researchers to help further their study in this field.

Autoren

Herausgeber

Smith, Sinead M

Inhaltsverzeichnis

1. An Overview of Helicobacter pylori InfectionRebecca FitzGerald and Sinéad Smith 2. The Urea Breath Test for the Non-Invasive Detection of Helicobacter pyloriAnthony O'Connor 3. Real-time Diagnosis of Helicobacter pylori during Endoscopy by Gastric Juice AnalysisCristiano Spada and Stefania Piccirelli 4. Molecular Detection of Antibiotic-resistant Helicobacter pylori Denise Brennan, Colm O'Morain, Deirdre McNamara, and Sinéad Smith 5. Endoscopic Detection of Helicobacter pylori by the Rapid Urease TestVikrant Parihar and Deirdre McNamara 6. Culture-based Antimicrobial Susceptibility Testing for Helicobacter pyloriPhilippe Lehours and Francis Mégraud 7. Purification of Total RNA from Stomach Tissue BiopsiesRebecca FitzGerald, Malini Devapal, John J. Hickey, Dermot Kelleher, and Sinéad Smith 8. Gene Expression Analysis by Reverse Transcription Quantitative PCR Eva M. Campion and Sinéad T. Loughran 9. A Method to Investigate the Helicobacter pylori-associated DNA MethylomeHae Dong Woo and Zdenko Herceg 10. Profiling the Gastrointestinal MicrobiotaBrunella Posteraro, Flavio De Maio, and Antonio Gasbarrini 11. Helicobacter pylori Virulence Factor GenotypingRebecca FitzGerald, Chavi Sinha, Abbas Yadegar, and Sinéad Smith 12. Generation of Isogenic Mutant Strains of Helicobacter pyloriStephanie L. Servetas, Jeannette M. Whitmire, and D. Scott Merrell 13. Isolation of Outer Membrane Vesicles from Helicobacter pylori Henry J. Windle 14. A Mouse Model of Helicobacter pylori InfectionTanmoy Kumar Dey, Bipul Chandra Karmakar, Avijit Sarkar, Sangita Paul, and Asish Kumar Mukhopadhyay 15. Investigating the Interaction of Helicobacter pylori with the Gastric MucosaMarguerite Clyne, Ciara Dunne, and Brendan Dolan 16. Evaluating Host Responses to Helicobacter pylori using ELISA and Western BlotCiara Nulty, Gillian Barber, Ewelina Flis, and Emma M. Creagh 17. Analysis of Innate Immune Responses to Helicobacter pyloriMichelle Chonwerawong and Richard L. Ferrero 18. Analysis of T Cell Responses to Helicobacter pylori InfectionKazuyo Kaneko and Karen Robinson 19. Miniaturised In Vitro Assays to Study Cellular Phenotypic Characteristics: Proliferation, Migration, Invasion, and Anoikis-resistanceNiamh McNamee and Lorraine O'Driscoll

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