Practical HPLC Method Development

2. Revised Auflage 1997
ISBN: 978-0-471-00703-6
Verlag: Wiley

Buch, Englisch, 800 Seiten, Format (B × H): 161 mm x 240 mm, Gewicht: 1343 g

This revision brings the reader completely up to date on the evolving methods associated with increasingly more complex sample types analyzed using high-performance liquid chromatography, or HPLC. The book also incorporates updated discussions of many of the fundamental components of HPLC systems and practical issues associated with the use of this analytical method. This edition includes new or expanded treatments of sample preparation, computer assisted method development, as well as biochemical samples, and chiral separations.

Autoren/Hrsg.

Snyder, Lloyd R

Kirkland, Joseph J

Glajch, Joseph L

Fachgebiete

• Interdisziplinäres Wissenschaften Wissenschaften: Forschung und Information Forschungsmethodik, Wissenschaftliche Ausstattung
• Naturwissenschaften Chemie Chemie Allgemein Chemometrik, Chemoinformatik
• Technische Wissenschaften Technik Allgemein Modellierung & Simulation
• Naturwissenschaften Chemie Analytische Chemie Instrumentelle Analytik, Chromatographie
• Naturwissenschaften Physik Physik Allgemein Experimentalphysik
• Naturwissenschaften Chemie Chemie Allgemein Toxikologie, Gefahrstoffe, Sicherheit in der Chemie
• Naturwissenschaften Chemie Chemie Allgemein Chemische Labormethoden, Stöchiometrie

Inhaltsverzeichnis

Diese sorgfältig überarbeitete, bedeutend erweiterte Auflage des Bestsellers zur Hochleistungs-Flüssigkeitschromatographie (HPLC) bringt dem Leser die neuesten methodischen Entwicklungen nahe. Aktualisiert wurden unter anderem die Kapitel zur Probenvorbereitung, zur computergestützten Methodenentwicklung und zu biochemischen Anwendungen.

Getting Started.

Basics of Separation.

Detection Sensitivity and Selectivity.

Sample Preparation.

The Column.

Non-Ionic Samples: Reversed- and Normal-Phase HPLC.

Ionic Samples: Reversed-Phase, Ion-Pair, and Ion-Exchange HPLC.

Gradient Elution.

Systematic Approach to the Reversed-Phase Separation of Regular Samples.

Computer-Assisted Method Development.

Biochemical Samples: Proteins, Nucleic Acids, Carbohydrates, and Related Compounds.

Chiral Separations.

Preparative HPLC Separation.

Quantitation (Including Trace Analysis).

Completing the Method: Validation and Transfer.

Appendices.

Index.

Über Autor(innen)

Lloyd R. Snyder, PHD, is a Principal at LC Resources in Walnut Creek, California. He is the author or coauthor of several books including An Introduction to Separation Science, Introduction to Modern Liquid Chromatography, Second Edition, and the bestselling Practical HPLC Method Development, Second Edition, all published by Wiley.

Joseph J. Kirkland, PhD, is Vice President of Research and Development for Advanced Materials Technology, Inc., and coauthor of Introduction to Modern Liquid Chromatography, Second Edition, Practical HPLC Method Development, Second Edition, and Modern Size-Exclusion Liquid Chromatography, Second Edition, all published by Wiley.

Joseph L. Glajch is the author of Practical HPLC Method Development, Second Edition, published by Wiley.