Buch, Englisch, Band 2398, 245 Seiten, Format (B × H): 178 mm x 254 mm, Gewicht: 496 g
Reihe: Methods in Molecular Biology
Methods and Protocols
Buch, Englisch, Band 2398, 245 Seiten, Format (B × H): 178 mm x 254 mm, Gewicht: 496 g
Reihe: Methods in Molecular Biology
ISBN: 978-1-0716-1914-8
Verlag: Springer US
This second edition provides new and updated protocols to investigate clock-controlled parameters including period estimations and entrainment measurements and modelling. Chapters detail promoter activity using luciferase reporters, fluorescent-tagging of clock proteins and cellular imaging, protein level measurements, regulated RNA splicing detection, post-translational modifications, protein-protein interactions, and protein DNA/chromatin interactions. Additional chapters focus on seasonal processes like hypocotyl growth and flowering time control in annuals and trees, and techniques on soybean. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.
Authoritative and practical, Plant Circadian Networks: Methods and Protocols, Second Edition is designed not only for the chronobiology community dealing with circadian biology but also for the plant community in general.
“Period estimation and rhythm detection in timeseries data using BioDare2, the free, online, community resource” is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
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Weitere Infos & Material
1. Circadian Rhythm: Phase Response Curve and Light Entrainment
Xiaodong Xu, Li Yuan, and Qiguang Xie
2. Period Estimation and Rhythm Detection in Timeseries Data Using BioDare2, the Free, Online, Community Resource
Tomasz Zielinski, Johnny Hay, and Andrew J. Millar
3. Detection and Analysis of Circadian Rhythms Via Prompt Chlorophyll Fluorescence
Yuri Dakhiya and Rachel Green
4. High Spatial Resolution Luciferase Imaging of the Arabidopsis thaliana Circadian Clock
Mark Greenwood, Anthony J.W. Hall, and James C.W. Locke
5. Agrobacterium-mediated seedling transformation to measure circadian rhythms in Arabidopsis
Michael K. Y. Ting, Reimo Zoschke, and Michael J. Haydon6. Measurement of Luciferase Rhythms in Soybean Hairy Roots
Qiguang Xie, Yu Wang, Li Yuan, and Xiaodong Xu7. ODE (ordinary differential equation) Models for Plant Circadian Networks: What The Models Are, and How They Should Be Used
Jonathan W. Pitchford and Paula Avello Fernandez8. How to Detect QTLs in the Plant Circadian Clock
Muhammad Usman Anwer and Marcel Quint
9. Measuring Hypocotyl Length in Arabidopsis
James Ronald and Seth J. Davis
10. High Throughput Extraction and Enzymatic Determination of Sugars and Fructans In Fructan-Accumulating Plants
Kallyne A. Barros, Masami Inaba, Auxiliadora Oliveira Martins, and Ronan Sulpice
11. Simple Nuclei Preparation and Co-Immunoprecipitation Procedures for Studying Protein Abundance and Interactions In Plant Circadian Time Courses
Akane Kubota, Young Hun Song, and Takato Imaizumi
12. Chromatin Immunoprecipitation Protocol for Circadian Clock Proteins
Sally Adams and Isabelle A. Carré
13. A high-throughput 3'-Tag RNA Sequencing for Large-Scale Time-Series Transcriptome Studies
Xiaoyu Weng and Thomas E. Juenger
14. Experimental Design for Time-Series RNA-seq Analysis of Gene Expression and Alternative SplicingNikoleta A. Tzioutziou, Allan B. James, Wenbin Guo, Cristiane P. G. Calixto, Runxuan Zhang, Hugh G. Nimmo, and John W. S. Brown
15. Using tandem affinity purification to identify circadian clock protein complexes from ArabidopsisMaria L. Sorkin and Dmitri A. Nusinow
16. Firefly Luciferase Complementation-Based Analysis of Dynamic Protein-Protein Interactions under Diurnal and Circadian Conditions in Arabidopsis
Qiguang Xie, Qiao Wang, and Xiaodong Xu
17. Monitoring Seasonal Bud Set, Bud Burst, and Cold Hardiness in Populus
Mikael Johansson, Naoki Takata, Cristian Ibáñez, and Maria E. Eriksson
18. The Perennial Clock Is an Essential Timer for Seasonal Growth Events and Cold Hardiness
Mikael Johansson, Cristian Ibáñez, Naoki Takata, and Maria E. Eriksson