Buch, Englisch, Band 2911, 200 Seiten, Format (B × H): 183 mm x 260 mm, Gewicht: 611 g
Reihe: Methods in Molecular Biology
Methods and Protocols
Buch, Englisch, Band 2911, 200 Seiten, Format (B × H): 183 mm x 260 mm, Gewicht: 611 g
Reihe: Methods in Molecular Biology
ISBN: 978-1-0716-4449-2
Verlag: Humana
This volume details Agrobacterium strain methods, such as plant genotype, transient and stable transformation of plants for gene overexpression, silencing, transplastomic and editing. Transformation protocols are focused mainly in plants with economic importance such as cereals and fruit trees including diploids and polyploids, and in non-plant species such fungi and algae. Written in the highly successful series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.
Zielgruppe
Professional/practitioner
Autoren/Hrsg.
Weitere Infos & Material
Preparation of chemocompetent Molecular analysis of Agrobacterium tumefaciens transformed strains with commercially available Plasmid DNA Miniprep kits.- Using Agrobacterium tumefaciens to assemble multi-step metabolic pathways in Nicotiana benthamiana.- Verifying plasmid constructs via transient Agrobacterium tumefaciens-mediated plant transformation in Nicotiana benthamiana.- Vacuum-Assisted Transient Agrobacterium tumefaciens-Mediated Transformation for plant molecular analysis.- Agrobacterium tumefaciens-mediated plant transformation and gene editing in rice.- Agrobacterium-Mediated Transformation for Commercial Wheat Varieties.- Agrobacterium-mediated transformation of Soybean (Glycine max L.) using split-cotyledonary explant.- Genetic transformation of Lettuce (Lactuca sativa) using Agrobacterium tumefaciens.- Agrobacterium tumefaciens-mediated genetic transformation of Nigella damascene.- Agrobacterium tumefaciens mediated stable cotyledon transformation of Solanum lycopersicum var Micro-Tom.- Agrobacterium-mediated transformation for gene editing tomato elite breeding lines.- Agrobacterium tumefaciens-mediated stable transformation of Malus domestica (Apple) cv Fuji RakuRaku and Royal Gala.- Agrobacterium tumefaciens-Mediated Transformation of Grape (Vitis vinífera sp).- Agrobacterium-Mediated Stable Transformation of Actinidia deliciosa (Kiwi).- Transformation of mature apricot (Prunus armeniaca L.) hypocotyl slices.- Agrobacterium-Mediated Transformation of Prunus persica (Peach) Immature Cotyledons.
