Subcellular Fractionation

Erscheinungsjahr 1997
ISBN: 978-0-19-963494-1
Verlag: OUP Oxford

Buch, Englisch, 360 Seiten, Print PDF, Format (B × H): 156 mm x 234 mm, Gewicht: 547 g

Many investigations into the structure and function of cells and tissues require the isolation of a particular membrane or subcellular component (organelle). This book covers all the necessary aspects, from breaking up the cells (homogenization), via a variety of separation techniques (the isolation and fractionation chapters), to characterization of the separated organelles.

Autoren/Hrsg.

Graham

Rickwood

Fachgebiete

• Naturwissenschaften Biowissenschaften Zellbiologie
• Naturwissenschaften Biowissenschaften Molekularbiologie

Inhaltsverzeichnis

- 1.: Homogenization of tissues and cells

- 2.: Isolation of subcellular fragments

- 3.: Isolation and characterization of nuclei and nuclear subfractions

- 4.: Subcellular fractionation of mitochondria

- 5.: Isolation and characterization of peroxisomes

- 6.: Lysosomes and endocytosis

- 7.: The membranes of the secretory and exocytic pathways

- 8.: Isolation and purification of functionally intact mitochondria and chloroplasts from plant cells

- 9.: Ribosomes and polysomes

- 10.: Electron microscopy of organelles