Buch, Englisch, Band 2544, 281 Seiten, Paperback, Format (B × H): 178 mm x 254 mm, Gewicht: 561 g
Reihe: Methods in Molecular Biology
Methods and Protocols
Buch, Englisch, Band 2544, 281 Seiten, Paperback, Format (B × H): 178 mm x 254 mm, Gewicht: 561 g
Reihe: Methods in Molecular Biology
ISBN: 978-1-07-162559-0
Verlag: Springer US
Authoritative and cutting-edge, Hepatocytes: Methods and Protocols aims to provide techniques to further understand molecular mechanisms involved in the regulating hepatocyte functions and underlying liver diseases.
Zielgruppe
Professional/practitioner
Autoren/Hrsg.
Fachgebiete
Weitere Infos & Material
Long-Term Expansion of Primary Hepatocyte Organoids.- Generation of in vivo traceable hepatocyte-like cells from human iPSCs.- Generation of hepatocyte organoids from human iPS cells.- Induction of bile canaliculi forming hepatocytes from human induced pluripotent stem cells.- Human hepatocyte transduction with adeno-associated virus vector.- Hepatocyte-directed delivery of Lipid-encapsulated small interfering RNA.- Co-culture model for hepatitis B virus infection using iPSC-derived liver progenitor cells and liver sinusoidal endothelial cells.- In vitro assay system to detect drug-induced bile acid-dependent cytotoxicity using hepatocytes.- Evaluation of urea cycle activity by metabolic flux analysis using mass spectrometry.- Live Cell Imaging of Biosynthetic Protein Transport in Hepatocytes.- Collagen sandwich culture of primary hepatocytes for image-based investigations.-Assessment of Hepatocyte Ploidy by Flow Cytometry.- Isolation of small hepatocyte-like progenitor cells from retrorsine/partial hepatectomy rat livers by laser micro-dissection.- Application of EdU-based DNA synthesis assay to measure hepatocyte proliferation in situ during liver regeneration.- Quantification of clonal expansion of hepatocytes in normal and injured liver.- Evaluation of SOX9-positive hepatocytes in human liver specimens and mature mouse hepatocytes.- Identification of genes regulating hepatocyte injury by a genome-wide CRISPR-Cas9 Screen.- Identification of subtypes of HCC using bioinformatics and the hepatocyte differentiation model.- 3D culture of primary patient-derived hepatoblastoma tumoroids.- Preparation of functional heaptocytes.