Buch, Englisch, Band 942, 392 Seiten, Format (B × H): 183 mm x 260 mm, Gewicht: 9074 g
Reihe: Methods in Molecular Biology
Methods and Protocols
Buch, Englisch, Band 942, 392 Seiten, Format (B × H): 183 mm x 260 mm, Gewicht: 9074 g
Reihe: Methods in Molecular Biology
ISBN: 978-1-62703-118-9
Verlag: Humana Press
The discovery of RNA interference (RNAi) as a methodology for gene silencing has revolutionized biological research, providing an invaluable avenue for therapeutics, and small interfering RNA (siRNA) is the most common strategy utilized for enacting RNAi. siRNA Design: Methods and Protocols offers expertly crafted guidelines and protocols for the selection of siRNA targeting sequences, for the strategic incorporation of chemical modifications, and for advantageous structural modifications to the classic siRNA design. Protocols are provided for using endogenous cellular machinery to produce siRNA from optimized precursor short hairpin RNA (shRNA) and artificial microRNA (amiRNA) molecules. Strategies are also described for specific applications such as immunostimulatory siRNA that may provide therapeutic benefit against viral infections in mammals, the simultaneous targeting of multiple siRNAs, and siRNA-mediated crop virus resistance. The design of RNAi for gene silencing in embryonic, invertebrate, and plant systems requires a variety of unique approaches, several of which are described towards the end of this volume. Written for the highly successful Methods in Molecular Biology™ series, this work contains the kind of detailed description and implementation advice that guarantees successful results.
Authoritative and easy to use, siRNA Design: Methods and Protocols will provide researchers, educators, clinicians, and biotech specialists with a broad understanding of the issues in RNAi and how they can be overcome strategically through design.Zielgruppe
Professional/practitioner
Autoren/Hrsg.
Fachgebiete
Weitere Infos & Material
1. What Parameters to Consider and Which Software Tools to Use for Target Selection and Molecular Design of Small Interfering RNAs
Olga Matveeva
2. Methods for Selecting Effective siRNA Target Sequences Using a Variety of Statistical and Analytical Techniques
Shigeru Takasaki
3. Designing Functional siRNA with Reduced Off-Target Effects
Yuki Naito and Kumiko Ui-Tei
4. Design and Screening of siRNAs Against Highly Structured RNA Targets
Neda Nasheri, John Paul Pezacki, and Selena M. Sagan
5. Engineering Small Interfering RNAs by Strategic Chemical Modification
Jesper B. Bramsen and Jørgen Kjems
6. The Design, Selection and Evaluation of Highly Specific and Functional siRNA Incorporating Unlocked Nucleobase Analogs
Narendra Vaish and Pinky Agarwal
7. The Design, Preparation, and Evaluation of Asymmetric Small Interfering RNA for Specific Gene Silencing in Mammalian Cells
Chanil Chang, Sun Woo Hong, Pooja Dua, Soyoun Kim, and Dong-ki Lee
8. Design of Nuclease-Resistant Fork-Like Small Interfering RNA (fsiRNA)
Elena L. Chernolovskaya and Marina A. Zenkova
9. Designing Dual-Targeting siRNA Duplexes Having Two Active Strands that Combine siRNA and MicroRNA-Like Targeting
Pål Sætrom
10. Strategies for Designing and Validating Immunostimulatory siRNAs
Michael P. Gantier
11. Designing Efficient and Specific Endoribonuclease Prepared siRNAs (esiRNAs)
Vineeth Surendranath, Mirko Theis, Bianca H. Habermann, and Frank Buchholz
12. Short Hairpin RNA-Mediated Gene Silencing
Luke S. Lambeth and Craig A. Smith
13. Design of Lentivirally Expressed siRNAs
Ying Poi Liu and Ben Berkhout
14. Bi-Functional Short Hairpin RNA (bi-shRNA): Design and Pathway to Clinical Application
Donald D. Rao, Neil Senzer, Zhaohui Wang, Padmasini Kumar, Chris M. Jay, and John Nemunaitis
15. Design and Chemical Modification of Synthetic Short shRNAs as Potent RNAi Triggers
Anne Dallas and Brian H. Johnston
16. Production and Application of Long dsRNA in Mammalian Cells
Katerina Chalupnikova, Jana Nejepinska, and Petr Svoboda
17. Design of RNAi Reagents for Invertebrate Model Organisms and Human Disease Vectors
Thomas Horn and Michael Boutros
18. Construction of shRNA Expression Plasmids for Silkworm Cell Lines Using Single-Stranded DNA and Bst DNA Polymerase
Hiromitsu Tanaka
19. Designing Effective amiRNA and Multimeric amiRNA Against Plant Viruses
Muhammad Fahim and Philip J. Larkin
20. Downregulation of Plant Genes with MiRNA Induced Gene Silencing
Felipe Fenselau de Felippes
