Thiamine, Thiamine Pyrophosphate and Thiamine Pyrophosphate-Dependent Enzymes Part A

Erscheinungsjahr 2026
ISBN: 978-0-443-47328-9
Verlag: Elsevier Science & Technology

Buch, Englisch, Format (B × H): 152 mm x 229 mm

Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters. Each chapter is written by an international board of authors.

Autoren/Hrsg.

Weitere Mitwirkende

Charmantray, Franck

Doumèche, Bastien

Fachgebiete

• Naturwissenschaften Physik Angewandte Physik Biophysik
• Naturwissenschaften Biowissenschaften Biochemie (nichtmedizinisch)
• Naturwissenschaften Biowissenschaften Molekularbiologie

Inhaltsverzeichnis

Section 1: Thiamine metabolism and assays
1. Continuous directed evolution of thiazole synthase
Kristen Van Gelder, Edmar Ramos de Oliveira-Filho, Ulschan Bathe, Anuran Gayen and J Donato Garcia-Garcia
2. Evolutionary history of thiamine biosynthetic pathways in the domains of life and recent distribution and diversification: a bioinformatic approach
Marie-Anne Van Sluys, Henrique Moura-Dias and Naiara Toledo
3. Generation and characterization of thiamin-biofortified plants
Aymeric Goyer, David Handy and Keonny Cosek
4. Experimental approaches to study thiamine triphosphate and vitamin B1 derivative metabolism in plants
Kai Wang
5. ThiN as a versatile domain of transcriptional repressors and catalytic enzymes of thiamine biosynthesis
Julie A. Maupin-Furlow, Nicholas Cox and Katherine Weber
6. Thiamine deficiency on the oleaginous yeast Yarrowia lipolytica
Cong T. Trinh
7. Assessment of thiamine status in humans
Kyly Whitfield, Elizabeth Frank, Kerry S. Jones and Lucien Bettendorff
8. Assay of thiamine pyrophosphokinase activity
Lucien Bettendorff and Pierre Wins
9. Thiamine quantification in fish tissue samples by assaying transketolase activity
Katie Edwards
10. Engineered nanopore biosensor for thiamine quantification
Florian Leonardus Rudolfus Lucas, Carsten Wloka and Giovanni Maglia

Section 2: Riboswitches
11. Identification and characterisation of thiamine pyrophosphate (TPP) riboswitch in Elaeis guineensis
Zetty Norhana Binti Balia Yusof
12. Scaffold engineering enables high-resolution cryo-EM analysis of thiamine pyrophosphate riboswitch architecture and dynamics
Navtej Toor, Daniel Haack, Madison Maille and Boris Rudolfs
13. Design and applications of chimeric antisense oligonucleotides that bind thiamine pyrophosphate mRNAs as antibacterial agents
Robert Penchovsky
14. Functional analysis of thiamine pyrophosphate-responsive riboswitches using a specialized dual-luciferase reporter gene assay
Matthias Mack and Anna Hübenthal
15. Mapping small ligand-RNA interactions using transcriptome-wide sequencing
Willem A. Velema and Daphne van den Homberg
16. Monitoring structural dynamics of nascent bacterial riboswitches responding to TPP within transcriptional complexes
Daniel A. Lafontaine, Mirette Hashem and Carlos Penedo

Über Autor(innen)

Charmantray, Franck
Franck CHARMANTRAY is a researcher in Biocatalysis at the CNRS. He carries out his research work at the Institute of Chemistry in Clermont-Ferrand (ICCF, UMR6296, Clermont Auvergne University, France). In 2001, he obtained a PhD in medicinal chemistry focusing on ' A new family of DNA intercalating-alkylating agents ' under the supervision of Dr. M. Demuynck, Grenoble 1 University, France. He then changed his research theme to focus on Studies on Homocitrate Synthase and other Lysine Pathway Enzymes during a postdoctoral internship funded by a BBRSC Fellowship, and carried out under the supervision of Prof. D. Young, at the University of Sussex, Brighton, UK. He returned to France to carry out a 2-year industrial postdoctoral internship in Biocatalysis financed by Laboratoires Fournier and which focused on the chemoenzymatic synthesis of antithrombotics. He was then recruited at the ICCF as a CNRS researcher in 2004. His research themes concern Biocatalysis, and in particular the study and optimization of transketolase by in vitro evolution for the synthesis of rare sugars and analogues in particular. He works in close collaboration with Dr. Bastien Doumèche with whom he recently developed an electrochemical screening for microbial transketolase inhibitors identification.

Doumèche, Bastien
Claude Bernard Lyon 1 and ICBMS (Institut de Chimie et Biochimie Moléculaires et Supramoléculaires, UMR 5246 CNRS, France).After biochemistry and biology studies in Rouen (France), he obtained a master degree in Amiens on a biocatalysis topic, followed by a PhD at the RWTH-Aachen university (Germany) working on immobilized enzymes in two-phase systems under Prof. W. Hartemeier supervision. He joined the group of Dr. Rolland Furstoss and Dr. Alain Archelas at the CNRS in Marseille (France) for a post-doc on multi-gram scale biocatalysis using epoxide hydrolases. After a two-years temporary associate professor position at the university of Cergy-Pontoise (France) with Prof. Véronique Larreta-Garde on sol-gel transitions of protein-polysaccharides mixtures catalysed by enzymes, he became a lecturer at the University Lyon 1in 2006 where he developed original research in heterogeneous biocatalysis (ionic liquids) and more recently electrochemical screening assays using screen-printed electrodes for oxidoreductases (dehydrogenases, oxidases, laccases) and transketolases in collaboration with Dr. Franck Charmantray.