Buch, Englisch, Band 873, 313 Seiten, Format (B × H): 183 mm x 260 mm, Gewicht: 816 g
Reihe: Methods in Molecular Biology
Buch, Englisch, Band 873, 313 Seiten, Format (B × H): 183 mm x 260 mm, Gewicht: 816 g
Reihe: Methods in Molecular Biology
ISBN: 978-1-61779-793-4
Verlag: Humana Press
Considerable advances have taken place since the initial isolation and characterization of human embryonic stem (HES) cells; however, significant challenges remain before their potential for restoration and regeneration processes in patients can be realized. Understanding the diversity amongst HES cell lines and realizing the ability to isolate lines with robust differentiation potential remain difficult. In the Human Embryonic Stem Cells Handbook, experts in the field provide an assortment of protocols that have been used by various laboratories around the world so as to allow both novices and experienced investigators to compare and contrast different approaches to HES cell isolation and characterization with the hope that, from these protocols, researchers might standardize approaches for HES cell biology. Written in the Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.
Authoritative and accessible, Human Embryonic Stem Cells Handbook serves as a valuable reference for scientists pursuing this vital field and its enormous potential.Zielgruppe
Professional/practitioner
Autoren/Hrsg.
Fachgebiete
Weitere Infos & Material
1. Establishment of New Lines of Human Embryonic Stem Cells: Evolution of the Methodology
Ana Maria Fraga, Érica Sara Souza de Araújo, Raquel Stabellini, Naja Vergani, and Lygia V. Pereira
2. Successful Scale-Up and Quality Assessments of Human Embryonic Stem Cells for Cell Therapy: Challenges and Overview
Udaykumar Kolkundkar, Mohan Vemuri, Geetha.M.Swamilingiah, Shruthi Pal, and Jasmeet Kaur
3. Human Embryonic Stem Cells Derived in Xeno-Free Conditions
Amparo Galán Albiñana and Carlos Simón Vallés
4. Procedures for Derivation and Characterization of Human Embryonic Stem Cells from Odense, Denmark
Linda Harkness and Moustapha Kassem
5. Principles for Derivation of Human Embryonic Stem Cells
Mikael C.O. Englund, Catharina Ellerström, Katarina Andersson, Karin Noaksson, and Johan Hyllner
6. Derivation and Maintenance of Undifferentiated Human Embryonic Stem Cells
Guoliang Meng and Derrick E. Rancourt
7. Establishment of hESC Lines from the Inner Cell Mass of Blastocyst-Stage Embryos and Single Blastomeres of 4-Cell Stage Embryos
Ileana Mateizel, Mieke Geens, Hilde Van de Velde, and Karen Sermon
8. Analysis of LINE-1 Expression in Human Pluripotent Cells
Martin Muñoz-Lopez, Marta Garcia-Cañadas, Angela Macia, Santiago Morell, and Jose L. Garcia-Perez
9. Characterization and Gene Expression Profiling of Five Human Embryonic Stem Cell Lines Derived in Taiwan
Steven Shoei-Lung Li
10. Derivation of Human Embryonic Stem Cell Lines from Poor Quality Embryos
Ronak Shetty and Maneesha S. Inamdar
11. Derivation, Expansion, and Characterization of Human Embryonic Stem Cell Lines from Aneuploid Embryos
Juan-Carlos Biancotti and Neta Lavon
12. Mutated Human Embryonic Stem Cells for the Study of Human Genetic Disorders
Ahmi Ben-Yehudah, Mira Malcov, Tsvia Frumkin, and Dalit Ben-Yosef
13. Single-Cell Enzymatic Dissociation of hESC Lines OxF1--OxF4 and Culture in Feeder-Free Conditions
Frances A. Brook
14. Protocol for Expansion of Undifferentiated Human Embryonic and Pluripotent Stem Cells in Suspension
Hossein Baharvand, Mehran Rezaei Larijani, and Maryam Yousefi
15. Suspension Bioreactor Expansion of Undifferentiated Human Embryonic Stem Cells
Roman Krawetz and Derrick E. Rancourt
16. Derivation, Propagation, and Characterization of Neuroprogenitors from Pluripotent Stem Cells (hESCs and hiPSCs)
Khun-Hong Lie, Henry Chung, and Kuldip S. Sidhu
17. Comparison of Neural Differentiation Potential of Human Pluripotent Stem Cell Lines Using a Quantitative Neural Differentiation Protocol
Dezhong Yin, Tahereh Tavakoli, Wei-Qiang Gao, and Wu Ma
18. Array-Comparative Genomic Hybridization Characterization of Human Pluripotent Stem Cells
Aaron M. Elliott, Kristi A. Hohenstein Elliott, and Anja Kammesheidt
19. Chromatin Immunoprecipitation-Based Analysis of Gene Regulatory Networks Operative in Human Embryonic Stem Cells
Marc Jung and James Adjaye
20. Analysis of the Methylome of Human Embryonic Stem Cells Employing Methylated DNA Immunoprecipitation Coupled to Next Generation Sequencing (MeDIP-seq)
Christina Grimm and James Adjaye
21. Stable Isotope Labelling with Amino Acids in Cell Culture for Human Embryonic Stem Cell Proteomic Analysis
Linda Harkness, Tatyana A. Prokhorova, Moustapha Kassem, and Blagoy Blagoev
