Buch, Englisch, Band 2072, 247 Seiten, Format (B × H): 183 mm x 260 mm, Gewicht: 693 g
Reihe: Methods in Molecular Biology
Methods and Protocols
Buch, Englisch, Band 2072, 247 Seiten, Format (B × H): 183 mm x 260 mm, Gewicht: 693 g
Reihe: Methods in Molecular Biology
ISBN: 978-1-4939-9864-7
Verlag: Springer US
The objective of this volume is to detail current technologies associated with cereal genomics, providing a valuable resource for researchers working in breeding and molecular crop improvement programs. Chapters guide readers through high-throughput DNA extraction protocols, crop genetic resources, meta-Quantitative Trait Loci (QTL) analysis, association mapping, next-sequencing generation, transposable element-associated variation, transcriptomics analysis, epigenetic variation, identification of imprinted genes, noncoding RNAs (circular RNAs), genome editing technologies, and post-translational protein phosphorylation.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Cereal Genomics: Methods and Protocols to ensure successful results in the further study of this vital field.
Zielgruppe
Popular/general
Autoren/Hrsg.
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Weitere Infos & Material
1. A Low-Cost High-throughput Method for Plant Genomic DNA Isolation
Prateek Gupta, Hymavathi Salava, Yellamaraju Sreelakshmi, and Rameshwar Sharma
2. Cereal Genomics Databases and Plant Genetic Resources in Crop Improvement
Robert J Henry
3. Integrated Genomic Strategies for Cereal Genetic Enhancement: Combining QTL and Association Mapping
Anurag Daware, Swarup K. Parida, and Akhilesh K. Tyagi
4. Sequencing and Assembling Genomes and Chromosomes of Cereal Crops
Marcelo Helguera
5. Cost-effective Profiling of Mutator Transposon Insertions in Maize by Next-generation Sequencing
Xinyan Zhang, Meixia Zhao, and Damon Lisch
6. Relative Expression Analysis of target genes by using Reverse transcription-Quantitative PCR
Rocío LilianaGómez and Lorena NoeliaSendín
7. DNA Methylation and Transcriptomic Next Generation Technologies in Cereal Genomics
Cynthia G. Soto-Cardinault, Fátima Duarte-Aké, Clelia De-la-Peña , and Elsa Góngora-Castillo
8. Genome-wide Identification of Regulatory DNA Elements in Crop Plants
Zhanjie Li and Kai Wang9. Genome-wide Profiling of Histone Modifications with ChIP-seq
William A. Ricci, Laura Levin, and Xiaoyu Zhang
10. Whole-genome Bisulfite Sequencing and Epigenetic Variation in Cereal Methylomes
Taiji Kawakatsu
11. Genome-wide Identification of Allele-Specific Gene Expression in a Parent-of-Origin Specific MannerChen Chenand Kevin Begcy
12. Analysis of Epigenetic Modifications during Vegetative and Reproductive Development in Cereals using Chromatin Immunoprecipitation (ChIP)
Kevin Begcy and Thomas Dresselhaus
13. Cereal Circular RNAs (circRNAs): An Overview of the Computational Resources for Identification and Analysis
Luis María Vaschetto, Celso Gaspar Litholdo Jr., Lorena Noelia Sendín, Claudia Mabel Terenti, and María Paula Filippone14. Emerging Genome Engineering Tools in Crop Research and Breeding
Andriy Bilichak, Daniel Gaudet, and John Laurie
15. CRISPR/Cas9-mediated Targeted Mutagenesis in Wheat Doubled Haploids
Alison M. R. Ferrie, Pankaj Bhowmik, Nandhakishore Rajagopalan, and Sateesh Kagale
16. Genetic Transformation of Protoplasts Isolated from Leaves of Lolium temulentum and Lolium perenne
Huw R. Davis, Anne L. Maddison, Dylan W. Phillips, and Huw D. Jones
17. Agrobacterium Transformation in the Rice Genome
Asuka Nishimura
18. In vivo Phosphorylation: Development of Specific Antibodies to Detect the Phosphorylated PEPC Isoform for the C Photosynthesis in Zea mays
Yoshihisa Ueno, Kumiko Yoshizawa-Kumagaye, Junji Emura, Tomoko Urabe, Taku Yoshiya, Tsuyoshi Furumoto, and Katsura Izui
