Capillary Electrophoresis of Carbohydrates

From Monosaccharides to Complex Polysaccharides
2011
ISBN: 978-1-62703-830-0
Verlag: Humana Press

Buch, Englisch, 277 Seiten, Paperback, Format (B × H): 155 mm x 235 mm, Gewicht: 446 g

Simple carbohydrates, complex oligosaccharides and polysaccharides all belong to a class of ubiquitous (macro)molecules that exhibit a wide range of biological functions, and the recent advent of enhanced enzymatic, chemical and analytical tools used to study these sugars has inaugurated a genuine explosion in the field of glycomics. Specifically, it has led to a deeper understanding of how specific sugar structures modulate cellular phenotypes, and that breakthrough has led to the discovery of new pharmaceuticals for the treatment of many serious diseases, such as cancer. The subsequent rapid expansion of this research holds high promise for future therapeutic regimens, and capillary electrophoresis (CE) refers to the range of related separation techniques that are integral to this vital research.

CE uses narrow-bore fused-silica capillaries to separate a complex array of large and small molecules, and Capillary Electrophoresis of Carbohydrates offers a comprehensive look at the latest breakthroughs and improvements in CE and CE techniques applied to monosaccharides up to complex oligosaccharides and polysaccharides. It begins with an overview of the application of CE and CE- mass spectrometric in the analysis of simple carbohydrates without any previous derivatization step before discussing various detection techniques such as spectrophotometric detection, electrochemical detection and other less common techniques. It then covers in detail an array of related topics and numerous applications. It is an essential text for anyone exploring the myriad possibilities of this rapidly expanding field.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Volpi, Nicola

Fachgebiete

• Naturwissenschaften Biowissenschaften Zellbiologie
• Naturwissenschaften Biowissenschaften Biochemie (nichtmedizinisch)
• Naturwissenschaften Biowissenschaften Kohlenhydrate

Inhaltsverzeichnis

Table of Contents.-.- PREFACE.-.- CHAPTER 1.- Analysis of simple carbohydrates by CE and CE-MS.- Christian W. Klampfl, Markus Himmelsbach, Wolfgang Buchberger.-.- CHAPTER 2.- Fluorophores and chromophores for the separation of carbohydrates by Capillary electrophoresis.- Michael Breadmore, Emily Hilder, Artaches Kazarian.-.- CHAPTER 3.- Capillary electrophoresis of bacterial (lipo)polysaccharides.- Nicola Volpi, Francesca Maccari.-.- CHAPTER 4.- Capillary electrophoresis and its microchip format for the analysis of glycosaminoglycans.- Kazuaki Kakehi, Yu-ki Matsuno.-.- CHAPTER 5.- Extracellular polysaccharides in microbial biofilm and their influence on the electrophoretic properties of microbial cells.- Filip Ruzicka, Marie Horka, Veronika Hola.-.- CHAPTER 6.- Capillary electrophoresis applied to polysaccharide characterization.- Mila Toppazzini, Anna Coslovi, Sergio Paoletti.-.- CHAPTER 7.- Capillary electrophoresis for monitoring natural and synthetic processes of saccharide bearing molecules.- Marco Rossi, Amelia Gamini, Cristiana Campa.-.- CHAPTER 8.- Analysis of intact glycoprotein biopharmaceuticals by CE.- Youssef Alahmad, Nguyet Thuy Tran, Myriam Taverna.-.- CHAPTER 9.- CE and CE-MS for structural analysis of N-glycans derived from glycoproteins.- Miyako Nakano, Kazuaki Kakehi, Naoyuki Taniguchi, Akihiro Kondo.-.- CHAPTER 10.- Monosaccharide compositional analysis of glycoproteins and glycolipids: utility in the diagnosis/prognosis of diseases.- Yehia Mechref.