A Practical Guide to Scanning Electron Microscopy in the Biosciences

1. Auflage 2022
ISBN: 978-3-527-35049-0
Verlag: Wiley-VCH GmbH

Buch, Englisch, 416 Seiten, Format (B × H): 188 mm x 241 mm, Gewicht: 1018 g

A concise and authoritative introduction to scanning electron microscopy in the biological sciences

In A Practical Guide to Scanning Electron Microscopy distinguished electron microscopist Gerhard Wanner delivers a practical handbook for biological scientists working with microbial, plant, and animal cells and tissues, enabling them to successfully apply scanning electron microscopy (SEM) to their object of study.

The book begins with an introduction to the principles of electron microscopy and the operation of electron microscopes before moving on to describe the preparation and mounting of specimens. It also explores the process of recoding images and their subsequent analysis, along with a wide range of advanced microscopy techniques, including cryo-SEM, FIB-SEM tomography, and stereo-SEM.

Scanning Electron Microscopy in the Biosciences contains hundreds of carefully selected microscopic images, as well as hands-on, step-by-step guidance required to perform a successful TEM experiment. Readers will also find: - Thorough introductions to optics, electron microscopy, electrons, and the components of electron microscopes
- In-depth examinations of the preparation of biological specimens and specimen mounting for scanning electron microscopy
- A comparison of different SEM modes and their strengths and weaknesses
- An introduction to novel techniques such as correlative light and electron microscopy (CLEM), array tomography, and cryo-scanning electron microscopy

Perfect for cell biologists and microbiologists, A Practical Guide to Scanning Electron Microscopy in the Biosciences also belongs in the libraries of neurobiologists and biophysicists.

Autoren/Hrsg.

Wanner, Gerhard

Fachgebiete

• Naturwissenschaften Physik Elektromagnetismus Mikroskopie, Spektroskopie
• Naturwissenschaften Physik Angewandte Physik Biophysik
• Naturwissenschaften Biowissenschaften Biowissenschaften
• Naturwissenschaften Chemie Chemie Allgemein Chemische Labormethoden, Stöchiometrie

Inhaltsverzeichnis

1. Introduction
2. Optics
3. Electron Microscopy
4. The Electrons
5. Components of the Electron Microscope
6. Preparation of Biological Specimens
7. Specimen Mounting for SEM
8. Microscopy
9. Resolution
10. Analytics
11. X-Rays
12. FIB/SEM
13. Serial Block Face SEM
14. Array Tomography
15. Cryo-SEM
16. VP-SEM
17. Stereo-SEM

Über Autor(innen)

Gerhard Wanner studied biology at Ludwigs Maximilian University in Munich (Germany), where he obtained his habilitation in 1992 and is now an Emeritus Professor of Ultrastructural Analysis at the Institute of Botany.
His main research interest is the analysis of chromosomes using scanning electron microscopy. He is the author of a highly successful textbook on plant microscopy (in German language).