Buch, Englisch, 468 Seiten, Format (B × H): 156 mm x 234 mm, Gewicht: 708 g
Principles, Methods, and Applications, Second Edition
Buch, Englisch, 468 Seiten, Format (B × H): 156 mm x 234 mm, Gewicht: 708 g
ISBN: 978-0-8493-1488-9
Verlag: CRC Press
Given the explosive development of new molecular marker techniques over the last decade, newcomers and experts alike in the field of DNA fingerprinting will find an easy-to-follow guide to the multitude of techniques available in DNA Fingerprinting in Plants: Principles, Methods, and Applications, Second Edition. Along with step-by-step annotated protocols, the authors fully discuss the technical aspects and modifications of existing techniques, the influence of reaction components and conditions, and the analysis of the results.
This second edition has been completely revised to address the exponential changes in the field since the first edition, focusing on PCR-based techniques but also including more sophisticated ones. The authors include numerous case studies to illustrate applications of the methods, more than 1600 references to the literature, and descriptions of reagent formulation, equipment, and computer programs used for evaluating molecular marker data. They compare the various methods, including the costs and benefits of each, helping readers determine which is best suited to a particular application.
The well-rounded, cross-referenced, and unified nature of this book makes it intrinsically easier to follow than the edited, multi-authored books currently available. It is an absolute necessity on the lab bench of anyone involved in plant research, DNA profiling, and molecular markers.
Zielgruppe
Academic, Professional, and Professional Reference
Autoren/Hrsg.
Fachgebiete
Weitere Infos & Material
Preface. Repetitive DNA: An Important Source of Variation in Eukaryotic Genomes. Detecting DNA Variation by Molecular Markers. Laboratory Equipment. Methodology. Evaluation of Molecular Marker Data. Applications of DNA Fingerprinting in Plant Sciences. Linkage Analysis and Genetic Maps. Which Marker for What Purpose: A Comparison. Future Prospects: SNiPs and Chips for DNA and RNA Profiling. Appendix 1: Plant DNA Isolation Protocols. Appendix 2: Suppliers and Sellers of Reagents and Equipment. Appendix 3: Computer Programs Dealing with the Evaluation of DNA Sequence Variation and Molecular Marker Data. Appendix 4: Web Pages of Interest. References. Index.
