High Throughput Bioanalytical Sample Preparation

Methods and Automation Strategies
Erscheinungsjahr 2003
ISBN: 978-0-444-51029-7
Verlag: Elsevier BV

Buch, Englisch, 640 Seiten, Format (B × H): 162 mm x 230 mm, Gewicht: 1139 g

High Throughput Bioanalytical Sample Preparation: Methods and Automation Strategies is an authoritative reference on the current state-of-the-art in sample preparation techniques for bioanalysis. This book focuses on high throughput (rapid productivity) techniques and describes exactly how to perform and automate these methodologies, including useful strategies for method development and optimization. A thorough review of the literature is included within each of these chapters describing high throughput sample preparation techniques: protein removal by precipitation; equilibrium dialysis and ultrafiltration; liquid-liquid extraction; solid-phase extraction; and various on-line techniques.The text begins with an introductory overview of the role of bioanalysis in pharmaceutical drug development. Fundamental understanding of the strategies for sample preparation is reinforced next, along with essential concepts in extraction chemistry. Several chapters introduce and discuss microplates, accessory products and automation devices. Particular strategies for efficient use of automation within a bioanalytical laboratory are also presented. The subject material then reviews protein precipitation, liquid-liquid extraction, solid-phase extraction and various on-line sample preparation approaches. The book concludes with information on recent advances in sample preparation, such as solid-phase extraction in a card format and higher density extraction plates.Important objectives that can be accomplished when the strategies presented in this book are followed include: improved efficiency in moving discovery compounds to preclinical status with robust analytical methods; return on investment in automation for sample preparation; and improved knowledge and expertise of laboratory staff.

Autoren/Hrsg.

Wells, David

Fachgebiete

• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Medizinische Fachgebiete Pharmakologie, Toxikologie
• Naturwissenschaften Chemie Analytische Chemie
• Naturwissenschaften Chemie Physikalische Chemie

Inhaltsverzeichnis

Chapter 1. Role of Bioanalysis in Pharmaceutical Drug DevelopmentChapter 2. Fundamental Strategies for Bioanalytical Sample PreparationChapter 3. High Throughput Tools for Bioanalysis: MicroplatesChapter 4. High Throughput Tools for Bioanalysis: Accessory ProductsChapter 5. Automation Tools and Strategies for BioanalysisChapter 6. Protein Precipitation: High Throughput Techniques and Strategies for Method DevelopmentChapter 7. Protein Precipitation: Automation StrategiesChapter 8. Liquid-Liquid Extraction: High Throughput TechniquesChapter 9. Liquid-Liquid Extraction: Strategies for Method Development and OptimizationChapter 10. Liquid-Liquid Extraction: Automation StrategiesChapter 11. Solid-Phase Extraction: High Throughput TechniquesChapter 12. Solid-Phase Extraction: Strategies for Method Development and OptimizationChapter 13. Solid-Phase Extraction: Automation StrategiesChapter 14. On-Line Sample Preparation: High Throughput Techniques and Strategies for Method DevelopmentChapter 15. Advances in Sample Preparation for Bioanalysis

Über Autor(innen)

Wells, David
David A. Wells, PhD, is Founder and Principal Scientist of Wells Medical Research Services (formerly Sample Prep Solutions), in Laguna Hills, California, USA. Dr. Wells is a researcher, author, consultant, and pharmacist with over 35 years of experience in the pharmaceutical industry, navigating the entire product lifecycle from bioanalytical discovery to post-marketing activities. His expertise has been developed in analytical chemistry, sample preparation, drug analysis, automation, medical information, medical writing, drug safety, medical communications, drug metabolism, toxicology, pharmacology, technical writing, product development and commercialization, technical service, and academic teaching.

Dr. Wells developed Empore™ disk-based sample preparation products for bioanalytical applications at 3M Corporation and managed all technical service activities for the program. He is a highly regarded author and educator whose body of work includes an authoritative reference book on drug sample preparation (High Throughput Bioanalytical Sample Preparation: Methods and Automation Strategies; Elsevier; 2003), 21 publications in scientific journals, six book chapters, and one patent.