Study of Bacteriorhodopsin in a Controlled Lipid Environment

Softcover Nachdruck of the original 1. Auflage 2019
ISBN: 978-981-13-4589-0
Verlag: Springer Nature Singapore

Buch, Englisch, 139 Seiten, Previously published in hardcover, Format (B × H): 155 mm x 235 mm, Gewicht: 254 g

Reihe: Springer Theses

This book focuses on the study of how the properties of nanodiscs, such as lipid composition and size, influence the function of the embedding integral membrane protein, bacteriorhodopsin. The author performed systematic studies to show that the lipid composition and the charge of the hydrophobic head and the structure of hydrophilic tails affect the photocycle pathway of bacteriorhodopsin, which is closely associated with its proton-pumping activity. Furthermore, the author demonstrated a highly efficient method for extracting membrane proteins directly from the biological membrane, preserving protein conformation, function and essential native lipids. This book demonstrates optimization and sample preparation, and presents practical methods of preparing membrane protein-embedded nanodisc samples for biophysical studies, which benefit structural and functional studies in the field of membrane protein characterization, both.

Zielgruppe

Research

Autoren/Hrsg.

Yeh, Vivien

Fachgebiete

• Naturwissenschaften Biowissenschaften Biochemie (nichtmedizinisch)
• Naturwissenschaften Physik Angewandte Physik Biophysik
• Naturwissenschaften Chemie Physikalische Chemie
• Naturwissenschaften Biowissenschaften Zellbiologie

Inhaltsverzeichnis

Introduction.- Experimental Background.- Effect of Lipid Composition of Nanodisc.- Effect of Size of Nanodisc.- Native Membrane Nanodisc.- Conclusions and Outlook.- References.- Appendix.