Buch, Englisch, 432 Seiten, Format (B × H): 162 mm x 236 mm, Gewicht: 1780 g
Reihe: Methods in Molecular Biology
Buch, Englisch, 432 Seiten, Format (B × H): 162 mm x 236 mm, Gewicht: 1780 g
Reihe: Methods in Molecular Biology
ISBN: 978-1-58829-725-9
Verlag: Humana Press
At the heart of most high-throughput methods is the technique of polymerase chain reaction (PCR). This book focuses on primer design, which is critical to both the efficiency and the accuracy of the PCR. With intricate descriptions of basic approaches as well as specialized methods, "PCR Primer Design" is an exceptional reference for all those involved in studying the genome.
Zielgruppe
Research
Autoren/Hrsg.
Fachgebiete
Weitere Infos & Material
Basic Principles and Software for PCR Primer Design.- Physical Principles and Visual-OMP Software for Optimal PCR Design.- OLIGO 7 Primer Analysis Software.- Selection for 3?-End Triplets for Polymerase Chain Reaction Primers.- The Reference Point Method in Primer Design.- PCR Primer Design Using Statistical Modeling.- Developing a Statistical Model for Primer Design.- Genome-Scale PCR Primer Design.- GST-PRIME.- Genome-Scale Probe and Primer Design with PRIMEGENS.- Repeat Masking for PCR Primer Design.- SNPbox.- Fast Masking of Repeated Primer Binding Sites in Eukaryotic Genomes.- Multiplex PCR Primer Design.- Degenerate Primer Design.- An Iterative Method for Selecting Degenerate Multiplex PCR Primers.- Primer Design for Multiplexed Genotyping.- MultiPLX.- MultiPrimer.- Allele-specific PCR.- Modified Oligonucleotides as Tools for Allele-Specific Amplification.- AlleleID.- Long PCR Primer Design.- Designing Primers for Whole Genome PCR Scanning Using the Software Package GenoFrag.- DNA Methylation Mapping.- Designing PCR Primer for DNA Methylation Mapping.- BiSearch.- Graphical Design of Primers with PerlPrimer.
