Advanced Methods in Molecular Biology and Biotechnology | E-Book | sack.de
E-Book

E-Book, Englisch, 218 Seiten

Advanced Methods in Molecular Biology and Biotechnology

A Practical Lab Manual

E-Book, Englisch, 218 Seiten

ISBN: 978-0-12-824450-0
Verlag: Elsevier Reference Monographs
Format: PDF
Kopierschutz: Adobe DRM (»Systemvoraussetzungen)



Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual is a concise reference on common protocols and techniques for advanced molecular biology and biotechnology experimentation. Each chapter focuses on a different method, providing an overview before delving deeper into the procedure in a step-by-step approach. Techniques covered include genomic DNA extraction using cetyl trimethylammonium bromide (CTAB) and chloroform extraction, chromatographic techniques, ELISA, hybridization, gel electrophoresis, dot blot analysis and methods for studying polymerase chain reactions. Laboratory protocols and standard operating procedures for key equipment are also discussed, providing an instructive overview for lab work. This practical guide focuses on the latest advances and innovations in methods for molecular biology and biotechnology investigation, helping researchers and practitioners enhance and advance their own methodologies and take their work to the next level.Explores a wide range of advanced methods that can be applied by researchers in molecular biology and biotechnologyFeatures clear, step-by-step instruction for applying the techniques coveredOffers an introduction to laboratory protocols and recommendations for best practice when conducting experimental work, including standard operating procedures for key equipment

Dr. Khalid Zaffar Masoodi, a young scientist working as an Assistant Professor at Division of Plant Biotechnology in Sher-e Kashmir University of Agricultural Sciences and Technology of Kashmir (India) and holds Masters and Ph.D in Biotechnology. After completing his Ph.D., he worked in Prof. Zhou Wang's Lab at University of Pittsburgh, PA, USA as Postdoctoral Associate (2010-2014). Dr. Masoodi is a recipient of SERB Early Career Research award 2016, Travel Award, SBUR, USA 2012, Society of Endocrinology Journal Award 2018 and many more. With a glorious academic career, Dr. Masoodi holds a distinction in qualifying CSIR-UGC net in Life Sciences, DBT-BET JRF and has qualified Graduate aptitude test in Engineering and Sciences (GATE) while he was doing his Masters in Plant Biotechnology.
His lab (Transcriptomics Laboratory, also called as K-Lab inspired by Klenow fragment that revolutionized Biotechnology research) at SKUAST-K is well equipped with Fluorescence imaging facility, RT-PCR, cell culture, Western blotting and many state-of-the-art instruments. His research focus is on multi-dimensional work on Transcriptomics of stress biology (biotic/abiotic) in plants and Cancer biology. Apart from that, he is extensively working on drug discovery from medicinal plants. He has produced high-impact papers published in Oncogene, Molecular Cancer Therapeutics, Endocrinology, Journal of Functional Foods, Plos One, and many more, and has over 230 sequences in Genbank. He also deciphered the whole transcriptome of six Kashmiri apple varieties. Currently, he has three externally funded projects from SERB, DST which focus on high throughput screening of Medicinal plants for prostate cancer and cisgenic apple. In 2019-20 Dr. Masoodi filed 5 patent applications with the Indian Patent office.
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