Alexander / Lundgren A Laboratory Manual of Analytical Methods of Protein Chemistry

Volume 4
1. Auflage 2014
ISBN: 978-1-4831-8564-4
Verlag: Elsevier Science & Techn.
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 246 Seiten, Web PDF

A Laboratory Manual of Analytical Methods of Protein Chemistry, Volume 4 provides information pertinent to the fundamental aspects of protein chemistry. This book discusses the simple and accurate methods of estimating specific proteins. Organized into six chapters, this volume begins with an overview of the composition of acids and experimental conditions for the acid hydrolysis of proteins. This text then examines the advantages of high-voltage electrophoresis for amino acid analysis, which are paralleled by equal advantages in the peptide separation field. Other chapters consider the simple technique of estimating specific proteins, which is one of several based on the phenomenon of antigen-antibody precipitation in gels. This book discusses as well the summations of analyses in weight percentages of the various residues and of the nitrogen of each constituent. The final chapter deals with the electrical properties of molecules. This book is a valuable resource for physicists and research workers.

Autoren/Hrsg.

Alexander, P.

Lundgren, H. P.

Inhaltsverzeichnis

1;Front Cover;1
2;A Laboratory Manual of
Analytical Methods
of Protein Chemistry;4
3;Copyright Page;5
4;Table of Contents;8
5;Dedication;6
6;PREFACE TO VOLUME 4;10
7;CHAPTER 1. THE ESTIMATION OF THIOL AND DISULPHIDE GROUPS;16
7.1;I. INTRODUCTION;16
7.2;II. TERMINOLOGY;17
7.3;III. PRINCIPLES;18
7.4;IV PROCEDURES;43
7.5;REFERENCES;84
8;CHAPTER 2. MICRO TECHNIQUES FOR AMINO ACID ANALYSIS AND PEPTIDE SEPARATION BASED ON HIGH-VOLTAGE ELECTROPHORESIS;91
8.1;1. INTRODUCTION;91
8.2;2. APPARATUS FOR HIGH-VOLTAGE ELECTROPHORESIS;93
8.3;3. THE ESTIMATION OF AMINO ACIDS;95
8.4;4. THE SEPARATION OF PEPTIDES;110
8.5;5. DISCUSSION;116
8.6;REFERENCES;118
9;CHAPTER 3. ESTIMATION OF SPECIFIC PROTEINS IN MIXTURES BY A GEL PRECIPITATION METHOD;121
9.1;INTRODUCTION;121
9.2;SUMMARY OF THE METHOD;124
9.3;ACCURACY;124
9.4;APPLICATIONS OF THE METHOD;124
9.5;THE METHOD;125
9.6;A MICRO VERSION OF THE METHOD;148
9.7;REFERENCES;149
10;CHAPTER 4. THERMAL POLYCONDENSATION OF a-AMINO ACIDS;152
10.1;INTRODUCTION;152
10.2;ANHYDROPOLYASPARTIC ACID POLYHYDRATE AND CONVERSION PRODUCTS;152
10.3;THERMAL COPOLYMERS OF TWO AMINO ACIDS;155
10.4;THERMAL POLYCONDENSATIONS OF LYSINE;162
10.5;PROTEINOIDS;164
10.6;POLYMERIZATION OF AMINO ACIDS WITH ORTHOPHOSPHORIC ACID AND WITH POLYPHOSPHORIC ACID;170
10.7;REFERENCES;173
11;CHAPTER 5. THE AMINO ACID COMPOSITION OF SELECTED PROTEINS AND POLYPEPTIDES;176
11.1;NOTES FOR TABLES II, III, AND IV;179
11.2;NOTES ADDED IN PROOF;184
11.3;REFERENCES;209
11.4;ADDENDUM;213
12;CHAPTER 6. DIELECTRIC MEASUREMENTS OF PROTEINS;215
12.1;A. INTRODUCTION;215
12.2;B. LOW-FREQUENCY MEASUREMENTS OF PROTEIN SOLUTIONS;221
12.3;C. HIGH-FREQUENCY MEASUREMENTS OF PROTEIN SOLUTIONS;239
12.4;D. MEASUREMENT OF SOLID PROTEINS;241
12.5;REFERENCES;243
13;AUTHOR INDEX;246
14;SUBJECT INDEX;252