Allemann / Scrutton Quantum Tunnelling in Enzyme-Catalysed Reactions

1. Auflage 2009
ISBN: 978-1-84755-997-5
Verlag: Royal Society of Chemistry
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band Volume 17, 412 Seiten

Reihe: RSC Biomolecular Sciences

In recent years, there has been an explosion in knowledge and research associated with the field of enzyme catalysis and H-tunneling. Rich in its breath and depth, this introduction to modern theories and methods of study is suitable for experienced researchers and those new to the subject. Edited by two leading experts, and bringing together the foremost practitioners in the field, this up-to-date account of a rapidly developing field sits at the interface between biology, chemistry and physics. It covers computational, kinetic and structural analysis of tunnelling and the synergy in combining these methods (with a major focus on H-tunneling reactions in enzyme systems). The book starts with a brief overview of proton and electron transfer history by Nobel Laureate, Rudolph A. Marcus. The reader is then guided through chapters covering almost every aspect of reactions in enzyme catalysis ranging from descriptions of the relevant quantum theory and quantum/classical theoretical methodology to the description of experimental results. The theoretical interpretation of these large systems includes both quantum mechanical and statistical mechanical computations, as well as simple more approximate models. Most of the chapters focus on enzymatic catalysis of hydride, proton and H" transfer, an example of the latter being proton coupled electron transfer. There is also a chapter on electron transfer in proteins. This is timely since the theoretical framework developed fifty years ago for treating electron transfers has now been adapted to H-transfers and electron transfers in proteins. Accessible in style, this book is suitable for a wide audience but will be particularly useful to advanced level undergraduates, postgraduates and early postdoctoral workers.

Autoren/Hrsg.

Allemann, Rudolf

Scrutton, Nigel S

Fachgebiete

• Naturwissenschaften Biowissenschaften Enzymologie
• Naturwissenschaften Chemie Physikalische Chemie Chemische Reaktionen, Katalyse

Inhaltsverzeichnis

The Transition-State Theory Description of Enzyme Catalysis for Classically Activated Reactions

Introduction to Quantum Behaviour – A Primer

Quantum Catalysis in Enzymes

Selected Theoretical Models and Computational Methods for Enzymatic Tunnelling

Kinetic Isotope Effects from Hybrid Classical and Quantum Path Integral Computations

Beyond Tunnelling Corrections: Full Tunnelling Models for Enzymatic C–H Activation Reactions

Quantum Effects in Enzyme Kinetics

Direct Methods for the Analysis of Quantum-Mechanical Tunnelling: Dihydrofolate Reductase

Probing Coupled Motions in Enzymatic Hydrogen Tunnelling Reactions: Beyond Temperature-Dependence Studies of Kinetic Isotope Effects

Computational Simulations of Tunnelling Reactions in Enzymes

Tunnelling does not Contribute Significantly to Enzyme Catalysis, but Studying Temperature Dependence of Isotope Effects is Useful

The Use of X-ray Crystallography to Study Enzymic H-tunnelling

The Strengths and Weaknesses of Model Reactions for the Assessment of Tunnelling in Enzymic Reactions

Long-Distance Electron Tunnelling in Proteins

Proton-Coupled Electron Transfer: The Engine that Drives Radical Transport and Catalysis in Biology

Über Autor(innen)

Nigel S. Scrutton graduated from King's College University of London before obtaining PhD and ScD degrees at the University of Cambridge. He is currently BBSRC Professorial Fellow and Professor of Enzymology at the University of Manchester. He has a longstanding interest in structural and mechanistic enzymology and has made a number of major contributions to the field of H-tunneling in biological systems. He has also been involved in interdisciplinary research programmes addressing the mechanisms of electron and hydrogen transfer in biology. Rudolf K. Allemann obtained a Dipl. Chem from the ETH in Zurich. He then studied for a PhD at Harvard University and the ETH-Zurich where he obtained Dr. sc. Nat. in 1988. He is currently a Research Professor and head of Chemical Biology at Cardiff University. His research interests focus on the physical and chemical basis of enzyme catalysis and structural and synthetic biology. He has also been involved in the de novo design of peptides that catalyze reactions with enzyme-like characteristics. Recently, he has pioneered the development of biophotonic nanoswitches for the control of protein-DNA and protein-protein interactions.