António Plant Metabolomics

Methods and Protocols
1. Auflage 2018
ISBN: 978-1-4939-7819-9
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 1778, 355 Seiten, eBook

Reihe: Methods in Molecular Biology

This detailed book aims to address the need for standardizing key steps in plant metabolomics research that is still considered a critical issue in the field.  The collection unites diverse mass spectrometry (MS)-based protocols, from sample preparation and extraction of plant primary and secondary metabolites (e.g. a range of sugars, lipids, phytohormones and volatile organic compounds) to metabolite analysis, using exclusively highly sensitive MS-based methodologies, followed by frontline bioinformatics and/or mathematical modeling approaches to produce reliable biological data interpretation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and practical, Plant Metabolomics: Methods and Protocols serves both new and well-established researchers in the plant metabolomics field in finding helpful guidelines and useful laboratory-based protocols for setting up their routine plant metabolomics experiments.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

António, Carla

Inhaltsverzeichnis

Part I: Review Chapters

1. The Importance of Experimental Design, Quality Assurance, and Control in Plant Metabolomics Experiments Marina C.M. Martins, Camila Caldana, Lucia Daniela Wolf, and Luis Guilherme Furlan de Abreu

2. Standard Key Steps in Mass Spectrometry-Based Plant Metabolomics Experiments: Instrument Performance and Analytical Method Validation Ana Margarida Rodrigues and Carla António

3. Guidelines for Sample Normalization to Minimize Batch Variation for Large-Scale Metabolic Profiling of Plant Natural Genetic Variance Saleh Alseekh, Si Wu, Yariv Brotman, and Alisdair R. Fernie

4. Gas Chromatography-Mass Spectrometry-Based 13C-Labeling Studies in Plant Metabolomics Valéria F. Lima, Leonardo P. Souza, Thomas C.R. Williams, Alisdair R. Fernie, and Danilo M. Daloso

5. Carbon Atomic Survey for Identification of Selected Metabolic Fluxes Leonardo Perez de Souza, Alisdair R. Fernie, and Takayuki Tohge

Part II: Mass Spectrometry-Based Methodology Chapters

6. Quantification of Low-Abundant Phosphorylated Carbohydrates Using HILIC-QqQ-MS/MS Tiago F. Jorge and Carla António

7. Quantification of Soluble Sugars and Sugar Alcohols by LC-MS/MS Regina Feil and John Edward Lunn

8. Untargeted Analysis of Semi-Polar Compounds by LC-MS and Targeted Analysis of Fatty Acids by GC-MS/GC-FID: From Plant Cultivation to Extract Preparation Camille Bénard, Sébastien Acket, Yannick Rossez, Olivier Fernandez, Thierry Berton, Yves Gibon, and Cécile Cabasson

9. Extraction of Plant Lipids for LC-MS-Based Untargeted Plant Lipidomics Thusitha W.T. Rupasinghe and Ute Roessner

10. Semi-Targeted Lipidomics of Plant Acyl Lipids Using UPLC-HR-MS in Combination with a Data-Independent Acquisition Mode Mohamed A. Salem and Patrick Giavalisco

11. Plant Lipidomics Using UPLC-QTOF-MS Yozo Okazaki and Kazuki Saito

12. Targeted LC-MS Analysis for Plant Secondary Metabolites Takafumi Shimizu, Mutsumi Watanabe, Alisdair R. Fernie, and Takayuki Tohge

13. An UHPLC–MS/MS Method for Target Profiling of Stress-Related Phytohormones Ondrej Novák and Kristýna Floková

14. A Complete Pipeline for Generating a High-Resolution LC-MS-Based Reference Mass Spectra Library Nir Shahaf, Asaph Aharoni, and Ilana Rogachev

15. Quantification of Sugars and Organic Acids in Biological Matrices Using GC-QqQ-MS Nirupama Samanmalie Jayasinghe, Himasha Mendis, Ute Roessner, and Daniel Anthony Dias

16. Acquisition of Volatile Compounds by Gas Chromatography-Mass Spectrometry (GC-MS) José G. Vallarino, Alexander Erban, Ines Fehrle, Alisdair R. Fernie, Joachim Kopka, and Sonia Osorio

17. Mass Spectrometry Imaging (MSI) for Plant Metabolomics Berin A. Boughton and Dinaiz Thinagaran

18. Laser Ablation Electrospray Ionization-Mass Spectrometry Imaging (LAESI-MS) for Spatially Resolved Plant Metabolomics Desalegn Etalo, Carmen Díez-Simón, Ric C.H. de Vos, and Robert D. Hall

19. Live Single-Cell Mass Spectrometry (LSC-MS) for Plant Metabolomics Keiko Masuda, Yasmine Abouleila, Ahmed Ali, Toshio Yanagida, and Tsutomu Masujima

Part III: Data Analysis & Statistics Chapters

20. Statistical and Multivariate Analysis of MS-Based Plant Metabolomics Data Carsten Jaeger and Jan Lisec

21. Integration of Plant Metabolomics Data into Metabolic Networks: Progresses and Challenges Nadine Töpfer, Samuel M.D. Seaver, and Asaph Aharoni

22. Understanding the Functionality of a Biological System as a Whole: Comparative Data Analysis Isabel Orf

23. Regression-Based Modeling of Complex Plant Traits Based on Metabolomics Data Francisco de Abreu e Lima, Lydia Leifels, and Zoran Nikoloski

24. Mathematical Modeling Approaches in Plant Metabolomics Lisa Fürtauer, Jakob Weiszmann, Wolfram Weckwerth, and Thomas Nägele